茯苓多糖的氧化及其活性研究

化工論文編號(hào):HG065  論文字?jǐn)?shù):9876,頁數(shù):25

 目  錄
中文摘要 I
英文摘要 II
目  錄 III
1  緒論 1
1.1茯苓簡介 1
1.2 茯苓多糖簡介 1
1.3 茯苓多糖的結(jié)構(gòu)修飾及研究進(jìn)展 2
1.4 茯苓多糖提取方法 4
1.5多糖的氧化 5
1.6茯苓多糖氧化物的生物活性檢測 6
1.7 實(shí)驗(yàn)思路 7
2.實(shí)驗(yàn)部分 1
2.1實(shí)驗(yàn)試劑與設(shè)備 1
2.1.1實(shí)驗(yàn)試劑 1
2.1.2 實(shí)驗(yàn)設(shè)備 1
2.2  實(shí)驗(yàn)步驟 2
3. 結(jié)果與討論 5
3.1 茯苓多糖氧化物的性能及產(chǎn)量 5
3.2 茯苓多糖氧化物的紅外譜圖 5
3.3 膽酸濃度與吸光度的標(biāo)準(zhǔn)曲線 6
3.4 膽酸結(jié)合能力試驗(yàn) 7
3.5 清除羥基自由基效果 8
3.6 氧化茯苓多糖分子量測定結(jié)果 9
4  結(jié)論與展望 1
4.1 結(jié)論 1
4.2 展望 1
致 謝 1
參考文獻(xiàn) 2

摘 要：本文主要研究茯苓多糖氧化物制備及活性研究。在TEMPO存在下將茯苓多糖進(jìn)行選擇性氧化制得氧化茯苓多糖，用傅立葉變紅外光譜對(duì)其進(jìn)行了結(jié)構(gòu)表征。同時(shí)對(duì)茯苓多糖氧化物的生物活性進(jìn)行了檢測，通過糠醛比色法測定了氧化茯苓多糖對(duì)膽酸的結(jié)合能力，發(fā)現(xiàn)茯苓多糖氧化物的膽酸結(jié)合能力比未經(jīng)化學(xué)修飾的茯苓多糖要高。利用Feton反應(yīng)產(chǎn)生羥基自由基，采用水楊酸顯色法測定茯苓多糖及其衍生物對(duì)羥基自由基的清除效果。在對(duì)自由基清除能力測試中發(fā)現(xiàn)，茯苓多糖氧化物對(duì)羥基自由基OH·和超氧自由基O2-·都有一定清除能力，且清除能力隨茯苓多糖氧化物加入的量的增加而增加。
 關(guān)鍵詞：茯苓多糖；氧化；紅外；膽酸結(jié)合能力；自由基清除能力

Study on the Oxidation of Pachymaran and Its Biological Activity

Abstract: This paper introduces the oxidation of pachymaran and its biological activity. During the preparation of the oxidation of pachymaran. TEMPO was used and then transform infrared spectroscopy was used to characterize the structure of oxidation product. At the same time, the biological activity of derivative was tested. According to furfural cuvette method, it was found that oxidation of pachymaran of bile acid binding capacity is higher than that without the chemical modification of pachymaran, but the overall bile acid binding capacity is not high. Using Feton reaction produces hydroxyl radical, it was found that oxidation of pachymaran have the effect of clearance to hydroxyl radical. In the free radical scavenging capacity test, it was found that the oxidation of pachymaran have some OH- hydroxyl radical and superoxide radical scavenging ability.

Key words: Pachymaran; Oxidation of pachymaran; FT-IR; Bile acid binding capacity; Free radical scavenging capacity;