響應面法優(yōu)化菜籽油甾醇提取

化工論文編號:HG210  論文字數(shù):12991,頁數(shù):30

 目  錄
中文摘要 I
英文摘要 II
目  錄 III
1. 緒論 1
1.1 植物甾醇的來源 1
1.2 植物甾醇的結構 1
1.3 植物甾醇的性質 1
1.4 植物甾醇的提取分離 1
1.4.1 溶劑結晶法 2
1.4.2 超臨界CO2萃取法 3
1.4.3 超聲波法 3
1.4.4 分子蒸餾法 3
1.4.5 絡合法 4
1.4.6 皂化法 4
1.5 植物甾醇的分析 5
1.5.1 毛地黃皂甙法 6
1.5.2 薄層色譜法(TLC) 6
1.5.3 可見光比色法(VS) 6
1.5.4 氣相色譜法(GC) 6
1.5.5 高效液相色譜法 7
1.6  植物甾醇的應用 7
1.6.1  醫(yī)藥工業(yè) 7
1.6.2 食品工業(yè) 7
1.6.3 化妝品業(yè) 8
1.6.4  化工、印刷等其它方面 8
1.7  植物甾醇的發(fā)展前景 8
2. 實驗部分 9
2.1  實驗原料、試劑與儀器 9
2.2  實驗步驟 9
2.2.1  甾醇的提取 9
2.2.2  甾醇的含量測定 9
3. 結果與討論 11
3.1  單因素考察及其分析 11
3.1.1  液料比對甾醇產(chǎn)量的影響 11
3.1.2  反應時間對甾醇產(chǎn)量的影響 11
3.1.3  萃取劑用量對甾醇產(chǎn)量的影響 12
3.1.4  反應溫度對甾醇產(chǎn)量的影響 13
3.1.5  萃取次數(shù)對甾醇產(chǎn)量的影響 13
3.1.6  其它實驗 14
3.2  響應面法優(yōu)化植物甾醇提取工藝-Box-Behnken法 15
3.3  響應面法確立最佳工藝條件 15
3.3.1  響應值結果及其擬合模型 15
3.3.2  等高線圖和響應面圖分析 18
3.3.3 模型的驗證 22
4.  總結與展望 23
致謝 24
參考文獻 25

摘 要：為優(yōu)化菜籽油植物甾醇的提取工藝，以10mL菜籽油的植物甾醇產(chǎn)量為指標，對液料比（V/V）、提取過程中反應時間、反應溫度、萃取劑用量及萃取次數(shù)等因素進行了考察。利用響應面法對提取植物甾醇的工藝進行優(yōu)化，在單因素實驗的基礎上，選取液料比，反應時間和萃取劑用量為隨機因子，根據(jù)中心組合設計原理采用三因素三水平的響應面分析法，依據(jù)回歸分析確定最優(yōu)提取工藝條件。結果表明，植物甾醇提取的最佳工藝條件為：液料比為4.3:1，反應時間為40min，反應溫度為93℃，用102mL的乙醚萃取，萃取1次。采用該工藝條件，10mL原油的甾醇產(chǎn)量可達到12.67mg。驗證實驗值為12.63mg，與理論值相對誤差為0.32%。最后對溶劑的回收進行研究。
 關鍵詞：菜籽油；植物甾醇；響應面分析法；工藝優(yōu)化

Abstract: The optimization experiment of the extraction of phytosterols from rapeseed crude oil was carried out and the optimal extraction condition was determined, and accordingly to be used in extracting of phytosterols. In the experiment extraction yield of phytosterol from 10mL rapeseed crude oil was taken as an index, the liquid-material ratio，the time of extraction, and the extractant volume., extraction temperature, and extraction times in the extraction process were studied.To optimize the extraction of phytosterols from Rapeseed crude oil using response surface methodology, basing on the single factor experiment, the response surface methodology with three factors at three levels is adopted according to the principle of central composite design. And the factors of optimum conditions for the extraction will be determined by regression analysis choosing the liquid-material ratio，the time of extraction, and the extractant volume. The result shows that the optimum conditions for the extraction are as follows: the liquid-material ratio is 4.3:1(V/V), the time of extraction is 40 min, the reaction temperature is 93℃，the extractant volume is 102mL,and the extraction times is once.Under the optimum conditons,the extraction yield of phytosterols from 10mL rapeseed crude oil is 12.67mg while the experimental data is 12.63 mg with relative error 0.23% compared with theoretical value.At last we studied the recycle of solvent.
 Keywords：Rapeseed crude oil；Phytosterols；Response surface methodology；
 Optimization