黃秋葵水提多糖工藝優(yōu)化

化工論文編號:HG058  論文字?jǐn)?shù):9549,頁數(shù):22

目  錄

中文摘要 I
英文摘要 II
目錄 III
1 緒論 1
1.1  前言 1
1.2  黃秋葵與多糖 1
1.2.1  黃秋葵基本情況 1
1.2.2 多糖的性質(zhì) 2
1.2.3 生物多糖的提取方法 3
2 試驗部分 6
2.1  主要試驗試劑與儀器 6
2.1.1 試驗試劑 6
2.1.2 試驗儀器 6
2.2  試驗方法 6
3  結(jié)果與討論 11
3.1  單因素試驗結(jié)果與討論 11
 3.2  正交試驗結(jié)果與討論 13
4 結(jié)論與展望 15
4.1 試驗結(jié)論 15
4.2 展望 15
致 謝 17
參考文獻 1

摘 要：本論文以浙江衢州產(chǎn)黃秋葵嫩莢果為原料，首先將其加工成凍干粉，然后利用水提的方法，經(jīng)由硫酸苯酚法來測取離心試樣溶液的吸光度，以此來測定黃秋葵凍干粉中的多糖含量。首先開展單因素試驗，分別通過對水提溫度、水提時間和料水比三個因素的比較，獲得各因素對多糖提取含量的影響。再設(shè)計正交試驗，改變水提時間、水提溫度和料水比三個變量，以獲得水提黃秋葵多糖的最佳試驗條件。研究得到的水法提取黃秋葵多糖的最佳試驗條件為：水提時間4小時，水提溫度50℃，料水比（凍干粉：蒸餾水）1：20。另根據(jù)試驗得知，水提次數(shù)越多，黃秋葵多糖的得率越高。
 
關(guān)鍵詞：黃秋葵；多糖；水提；正交

Optimization of Okra Polysaccharide by water extraction
Abstract: This article use Okra fruit pods as raw materials, processed into freeze-dried powder，using water extraction methods, by phenol sulfuric acid method for measurement of the absorbance of the sample solution was centrifuged, in order to determine okra polysaccharide content of freeze-dried powder. First of all, to carry out single factor experiments were provided by the water temperature, extraction time and ratio of water extraction of three factors are the impact on the polysaccharide. Then use orthogonal experimental method, by changing the extraction time, water extraction temperature, and the ratio to get the water extract the best experimental conditions for okra polysaccharide. Of water extraction by okra polysaccharide optimal experimental conditions: water extraction time of 4 hours, water extraction temperature 50℃, the ratio of water (freeze-dried powder: water) 1:20. According to experimental results, the more the number of water extraction, and the higher the yield of okra polysaccharide.

Keywords: Okra; polysaccharide; water extraction; orthogonal.