栝樓籽中活性成分分離及分析方法研究

化工論文編號:HG073  論文字數:9586,頁數:25

 目 錄

1  緒論 1
 1.1   栝樓的特性 1
 1.2  栝樓的食用特點和價值 1
 1.3  栝樓的藥理作用 2
 1.4   栝樓的經濟效益 3
 1.5   栝樓的開發前景 3
 1.6  角鯊烯 4
 1.7  栝樓籽油的制備法 6
 1.7.1索氏提取法 6
 1.7.2壓榨法 6
 1.8  高效液相色譜分析法 7
2  實驗部分 8
 2.1  實驗原料、試劑與儀器 8
 2.2  栝樓籽仁中粗脂肪的含量測定 8
 2.3  酸價的測定 9
 2.4  皂化值的測定 9
 2.5  色譜條件 10
 2.6. 用螺旋榨油機榨油 10
3  結果和討論 11
 3.1   標準曲線的繪制 11
 3.2  未經水化脫膠的油脂中角鯊含量測定 13
 3.3  經水化脫膠的油脂中角鯊烯含量測定 14
4  總結與展望 18
致  謝 19
參考文獻 20

摘　要：隨著科學技術的發展,近些年發現栝樓具有很好的醫療作用，因此,國內需求量和出口量增長很快,顯示出良好的發展勢頭。我國是栝樓傳統主產國之一,歷來國際信譽較高,只要好種植區域,并注意用生物防治法防病治蟲,限制農藥的使用種類、使用量以及使用時間,并及時作好商品檢驗和監督,人工栽培栝樓的生產前景是十分廣闊的。實驗對栝樓籽仁油脂的提取以及油脂的酸價、皂化值和角鯊烯含量進行了分析和測定。結果表明:栝樓籽仁酸價( KOH)為19.23mg/g，皂化值( KOH)為204.84mg/g,角鯊烯含量約為0.1%。確立色譜條件為：色譜柱型號：Hyperil ODSe，流動相為甲醇/乙腈:60/40、檢測波長為203nm、流速為2ml/min、柱溫為40℃。
 
 關鍵詞：角鯊烯；栝樓籽；成分分析；高效液相色譜
  
Separation of Active Components and Analysis Method of Trichosanthes Kiriowii
Abstract : Along with the development of trichosanthes kirilowii in recent years, trichosanthes kirilowii is found good medical effects, therefore, the domestic demand and output grows fast, shows a good development momentum. China is one of the trichosanthes kirilowii traditional production countries with high international prestige. As long as we choose the good planting area and pollution prevention with biological pesticide, limit pest prevention of species, usage and using time, make timely commodity inspection and supervision, the prospect of production of artificial cultivation trichosanthes kirilowii will be very wide.Trchosanthes kiriowii Maxim seed was used as material. Itcomponent s and it s oil properties were studied. The acidvalue( KOH) is 19.23mg/g,saponificationvalue(KOH) is 204.84mg/g, Squalene is 0.1%.The HPLC conditions are the best:column:Hyperil ODSe;
mobile phase，V(acetonitrile)/V（methano1）=60/40;detection wavelength:203nm;
flow rate,2ml／min;tmperature:40℃

Keywords : Squalene;Trchosanthes kiriowii Maxim;Component analysis;HPLC