正交試驗超聲優化茯苓多糖提取工藝

化工論文編號:HG104  論文字數:13305,頁數:30

 目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1 茯苓的概述 1
 1.2  茯苓多糖的概述 2
 1.3  茯苓多糖衍生物的作用 4
 1.4  超聲波的基本作用原理及在提取生物活性物中的作用 5
 1.5 稀堿浸提法 6
 1.6 研究思路 7
2. 實驗部分 8
 2.1 實驗原料、試劑及試驗儀器 8
     2.2 實驗原理 8
 2.3 實驗方法 9
3. 結果與討論 12
 3.1 波長的選擇 12
 3.2 標準曲線的制作 12
 3.3 換算因子的確定 13
  3.4 單因素分析 13
  3.5 提取工藝正交優化實驗 17
 3.6 茯苓多糖的紅外光譜分析 19
4. 總結與展望 21
     4.1結論 21
 4.2存在的問題 22
 4.3展望 22
致謝 23
參考文獻 24

摘 要：在單因素實驗的基礎上利用正交實驗設計考察堿法提取過程中堿濃度、提取時間、料液比對茯苓多糖提取率的影響，確定最佳提取工藝條件為 : NaOH濃度 0. 75mo l /L ,時間2min ,料液比 1∶40。在此工藝條件下茯苓多糖的提取率高達86%。
關鍵詞：茯苓多糖；超聲波；提取；正交試驗
 Study on the Extraction Technology of  Poria cocos  Polysaccharide under Ultrasound by Orthogonal Design

Abstract: With the assistance of ultrasound, the polysaccharide from Poria cocos wolf was extracted using NaOHaq. The influence of extraction conditions including extraction time, ratio of raw materials and NaOHaq(V), concentration of media were studied. Then the optimization of conditions of extraction by the orthogonal design was investigated, it was found that the optimum conditions were NaOH 0.75mol/L, extraction time 2min, materials/solution1:40. Under the best condition, the extraction ratio of polysaccharide from Poria cocos wolf was as high as 86%.
Key words： Poria cocos polysaccharide ; ultrasonic wave ; extraction ; orthogonal test