正交優(yōu)化菜籽油甾醇提取工藝

化工論文編號(hào):HG133  論文字?jǐn)?shù):8177,頁(yè)數(shù):22

 目  錄
中文摘要 I
英文摘要 II
目錄 III
1  緒論 1
 1.1  植物甾醇的結(jié)構(gòu)和理化性質(zhì) 1
 1.2  關(guān)于提取植物甾醇的研究 2
 1.3  植物甾醇應(yīng)用 3
 1.4  植物甾醇的開(kāi)發(fā)及研究現(xiàn)狀 5
2  實(shí)驗(yàn)部分 6
 2.1  實(shí)驗(yàn)原料，試劑與儀器 6
 2.2  實(shí)驗(yàn)步驟 6
3  結(jié)果與討論 8
 3.1  單因素考察 8
 3.2  正交實(shí)驗(yàn)結(jié)果及極差分析 11
 3.3  溶劑的回收 13
 3.4  對(duì)比實(shí)驗(yàn) 14
4  總結(jié)與展望 15
致謝 16
參考文獻(xiàn) 17

摘 要：對(duì)植物甾醇提取工藝進(jìn)行優(yōu)化，得出較佳提取條件，可應(yīng)用于植物甾醇的提取。以10mL菜籽油的植物甾醇產(chǎn)量為指標(biāo)，對(duì)萃取劑用量、提取過(guò)程中反應(yīng)溫度，萃取的次數(shù)，液料比，反應(yīng)時(shí)間等因素進(jìn)行了單因素考察，最后用正交法進(jìn)行優(yōu)化。實(shí)驗(yàn)結(jié)果表明：萃取劑用量60mL，反應(yīng)溫度93℃，提取次數(shù)1次，取時(shí)間為45min，萃取劑用量50mL, 液料比為5:1；溶劑回收再利用可行。通過(guò)正交設(shè)計(jì)得出較佳條件為：提取不皂化物時(shí)，加氫氧化鈉-乙醇溶液40mL，在93℃下皂化60min，再用110mL乙醚萃取1次。
 關(guān)鍵詞：菜籽油；甾醇；提取；正交優(yōu)化

Abstract: In this article the optimization experiment of the extraction of phytosterols from rapeseed crude oil was carried out and the optimal extraction condition was determined, and accordingly to be used in industrialization extracting of phytosterols. In the experiment extraction yield of phytosterol from 10mL rapeseed crude oil was taken as an index, extraction agent, extraction temperature, extraction times, solid-liquid ratio, and extraction time in the extraction process were studied, finally the experiment was optimized by orthogonal. The results showed that under the solid-liquid ratio was 5:1(V/V), the time of extraction was 45 min, the extractant volume was 50 mL，the temperature of extraction was 93℃, the extractant tim was 1, liquid ratio was 5:1; The solvent could be recycled. Obtained by orthogonal design, the better conditions were obtained by orthogonal design as follows: when extracted unsaponifiable matter, adding sodium hydroxide-ethanol 40mL, saponification at 93℃ under 60min, then 110mL ether extraction 1 time.
Keywords：Rapeseed crude oil；Phytosterols；Extraction；Orthogonal optimization