正交優化葛根黃酮提取工藝

化工論文編號:HG144  論文字數:11017,頁數:26
目  錄
中文摘要 I
英文摘要 II
目錄 III
1．緒論 1
1.1  葛根的種類及分布 1
1.2  葛根的化學成分 1
1.3  葛根的利用 2
1.3.3  葛根的保健作用 3
1.4  葛根黃酮的提取方法 4
1.5  葛根黃酮的檢測方法 4
1.6  葛根的發展前景 5
2．實驗部分 8
2.1  實驗原料、試劑與儀器 8
2.2  提取操作及分析過程 8
3．實驗結果與討論 9
3.1  標準曲線的繪制 9
3.2  單因素考察 10
3.3  正交設計 15
3.4  正交結果與分析 15
4．總結與展望 19
致謝 20
參考文獻 21

摘 要：對葛根黃酮提取工藝進行優化，得出較佳提取條件，可應用于葛根黃酮的工業化提取。實驗以葛根黃酮提取率為指標，對葛根黃酮提取過程中的溶劑乙醇質量分數、提取時間、固液比、提取溫度、pH、提取次數、重復性以及回收溶劑的再利用等因素進行了考察，最后用正交法進行優化。實驗結果表明：葛根黃酮的初步提取工藝為溶劑乙醇質量分數為70%，提取時間為120min，固液比為1:10，提取溫度為70℃，pH=5，提取次數1次；浸提操作重復性好，溶劑回收再利用可行并且溶劑的回收率高達94.3%。通過正交設計得出較佳條件為：乙醇質量分數為50%，提取時間為120min，固液比為1:12，提取溫度為80℃。
 關鍵詞：葛根黃酮；提取工藝；提取率；溶劑回收；正交設計

Abstract: The optimization experiment of puerarin flavonoids was carried out and the optimal extraction condition was determined, and accordingly to be used in industrialization extracting of puerarin flavonoids. In the experiment Puerarin flavonoids yield was taken as an index, mass fraction of ethanol, extraction time, solid-liquid ratio, extraction temperature, solution pH, extraction times, repeatability, recovery and reuse of solvent in the extraction process were studied, finally the experiment was optimized by orthogonal. The results showed that the mass fraction of ethanol was 70%, the extraction time was 120min, the solid-liquid ratio was 1:10, the extraction temperature was 70℃, the solution pH was 5, the extraction times was 1; repeatability for extraction operation was good, recycling solvents was feasible and the solvent recovery was up to 94.3%. The better conditions were obtained by orthogonal design as follows: the mass fraction of ethanol was 50%, the extraction time was 120min, the solid-liquid ratio was 1:12, the extraction temperature was 80℃.
 Keywords: Puerarin Flavonoids; Extraction process; Yield; Solvent recovery; Orthogonal design