響應面法優化葛根黃酮提取

化工論文編號:HG149  論文字數:12488,頁數:30

 目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
1.1  葛根介紹 1
1.2  葛根的主要有效組分 1
1.3  組分功效 2
1.4  葛根藥理作用 3
1.5  黃酮性質及效用 5
1.6  葛根黃酮的提取方法 6
1.7  葛根黃酮的檢測方法 7
1.8  葛根發展前景 7
2. 實驗部分 8
2.1  實驗原料、試劑與儀器 8
2.2  提取操作及分析過程 8
2.3  標準曲線繪制 9
3. 實驗結果與討論 11
3.1  單因素考察 11
3.2  響應面法優化葛根黃酮提取工藝Box-Behnken法 16
3.3  響應面法確立最佳工藝條件 16
4. 總結與展望 23
致謝 24
參考文獻 25

摘 要：為優化葛根黃酮提取工藝，以葛根黃酮提取率為指標，對葛根黃酮提取過程中溶劑乙醇質量分數、提取時間、固液比、提取溫度、pH、提取次數、重復性以及回收溶劑再利用等因素進行了考察。結果表明葛根黃酮的較優提取工藝為溶劑乙醇質量分數為70%，提取時間為120min，固液比為1:10，提取溫度為70℃，pH=5，提取次數1次；浸提操作重復性好，溶劑回收再利用可行并且溶劑的回收率高達94.3%。在此基礎上，通過Box-Behnken方法研究了固液比、提取溫度、溶劑乙醇質量分數對葛根黃酮提取率的影響，建立數學模型，以確定提取葛根黃酮的最優條件。通過模型的響應曲面圖及其等高線圖，對影響葛根黃酮提取率的關鍵因素及其相互作用進行探討，得到的優化工藝參數為：固液比為1:11，提取溫度為78℃，溶劑乙醇質量分數為66%。在上述條件下提取效果最佳，葛根黃酮的提取率為2839.78μg/g。
 關鍵詞：葛根黃酮；提取工藝；溶劑回收；響應面分析
 
Abstract: In order to optimize the extraction process of the Puerarin flavonoids,
Puerarin flavonoids yield was taken as an index, the quality score of solvent ethanol, extraction time,material-water ratio, extraction temperature,pH,extraction times, repeatability, recovery and reuse of solvent in the extraction process were studied. The results showed that the quality score of the ethanol was 70%, the extraction time was 120min, the material-water ratio was 1:10, the extraction temperature was 70℃, the pH was 5,the extraction times was 1; repeatability for extraction operation was good, recycling solvents was feasible and the solvent recovery was up to 94.3%. On this basis, the conditions of extracting Puerarin flavonoids were optimized using response surface mothod (RSM). Experimental factors and their levels were determined by one-factor tests.Subsequently, the Box-Benhnken experimental design with 3 factors and 3 levels was performed, and the factors influencing the technological were estimated by quadratic regression analysis with interaction terms. Response surface and contour were finally graphed with the extraction rate as the response value.The results showed that the optimal conditions of flavonoids extration were obtained as follows: the material-water ratio was 1:11, extraction temperature was 78℃，the quality score of solvent ethanol was 66 % and the extraction rate could be up to 2839.78μg/g.
Keywords: Puerarin Flavonoids; Extraction process; Solvent recovery;
 Response surface method