吡硫醇鋅檢測(cè)方法的建立

化工論文編號(hào):HG181  論文字?jǐn)?shù):9522,頁數(shù):23

 目 錄
中文摘要 ...................................................................I
英文摘要 ..................................................................II
目錄 ......................................................................Ⅲ
1. 緒論.....................................................................1
 1.1 ZPT簡介............................................1
 1.1.1 ZPT檢測(cè)方法...............................................1
 1.2高效液相色譜法檢測(cè)ZPT含量...........................4
2. 實(shí)驗(yàn)部分.................................................................5
 2.1 材料與實(shí)驗(yàn)儀器.........................................................5
 2.1.1  實(shí)驗(yàn)試劑與樣品..................................................5
 2.1.2  實(shí)驗(yàn)儀器........................................................5
 2.2 ZPT的含量檢測(cè)方法的建立...................6
 2.2.1 ZPT標(biāo)準(zhǔn)溶液配制...........................................6
  2.3 高效液相色譜法檢測(cè)ZPT的含量..............6
 2. 3. 1  ZPT的溶解度. . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . 6
 2.3.3 流動(dòng)相種類、配比、pH值的選擇...................................6
 2. 3. 4 最低檢出限測(cè)定. . . . . . . . . . . .. . . . . . .. . . . . . . . . . . 7
 2.3.2 精密度試驗(yàn).......................................................7
  2. 4高效液相色譜法銅鹽絡(luò)合法測(cè) ZPT的含量..............................7
3. 結(jié)果與討論...............................................................7
 3.1ZPT的含量檢測(cè)方法的建立....................7
 3.1.1 檢測(cè)波長的選擇...................................................7
 3.1.2 流動(dòng)相種類、配比、pH值的選擇.......................................11
 3.2 ZPT含量結(jié)果分析.......................... 11
 3.2.1 標(biāo)準(zhǔn)曲線的制作..................................................11
 3.2.2精密度試驗(yàn)...................................................12
 3.2.3 加標(biāo)回收率試驗(yàn).................................................. 13
 3.2.4最低檢出限測(cè)定......................................................13
4．總結(jié)與展望...............................................................14
致謝.......................................................................16
參考文獻(xiàn)...................................................................18

摘要：吡硫醇鋅 (Pyrithionc Zinc, ZPT) 的分子量為317.7，外觀是乳白色水分散體、灰白色粉末狀固體，具有較強(qiáng)地殺真菌作用，外用治療真菌感染，據(jù)報(bào)告用Z PT洗劑是很安全的。因局部可吸收部份藥物 (0.05%—0 .8％ )；此外吡硫醇鋅還有保鮮的效果。用 Z P T保鮮劑處理，可保持果實(shí)新鮮，好果率高，品質(zhì)優(yōu)良．可周年供應(yīng)。
 本文主要采用高效液相色譜法對(duì)ZPT含量進(jìn)行了檢測(cè)，此方法簡便、快速、結(jié)果可靠，可用于本品質(zhì)量控制。實(shí)驗(yàn)采用:Diamonsil Cl8 柱 ,5 μm ,（250mm ×4.6mm)色譜柱，經(jīng)改變流動(dòng)相類型、配比、pH值等因素，確定最佳流動(dòng)相配比為乙腈∶甲醇∶ 水溶液(10mmol\L 磷酸二氫鉀和0. 5mmol\l L EDTANa2 ,磷酸調(diào)pH=4.14) = 50∶ 10∶ 40，流速1.0 ml/min，在檢測(cè)波長230 nm下，得到標(biāo)準(zhǔn)曲線方程為Y=9299.5X-18030,R2=0.9997，加標(biāo)回收率為101.90%，RSD為0.96%，精密度為0.13%。
關(guān)鍵詞：高效液相色譜法（HPLC）；  ZPT；  吡啶硫酮銅絡(luò)合物；  含量檢測(cè)。

Abstract: The molecular weight of Pyrithionc Zinc is 317.7,  Appearance is a milky water spread、Gray solid. Having to kill a fungus role, With the treatment of fungal infection, Reported by z pt lotions is quite safe. For local can absorb some of the drug (0.05% - 0. 8 ％), The rich keep the sulphur is the effect of zinc. With z p t, can keep fresh fruit, fruit, high quality and can anniversary  provide.
   This article mainly adopt HPLC of zpt content of the testing . This method is simple, fast, reliable and can be validated to improve the quality control of ZPT. The chromatographic separation was performed on  a Diamonsil Cl8column(250 mm × 4.6 mm id, 5 μ m particle size). After several tests by changing the type of mobile phase, ratio and pH value, etc., a HPLC method using an elution with lAcetonitrile-Methano and 10mmol / L monopotassium phosphate and 0. 5mmol\l L EDTANa2  aqueous solution (pH=4.14, adjusted by phosphoric acid)( 50∶ 10∶40 ,V / V / V) at a flow-rate of 1.0 mL / min has been developed. At ultraviolet wavelength 230nm, the standard curve equation of zpt Y=9299.5X-18030, R2=0.9997, plus recovery of  101.90 percent , RSD to 0.96 %, precision of 0.13 % .
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 Key words:  high-performance liquid chromatography (HPLC); zinc pyrithione; Cu2+ complex of pyrithione  baicalin; Detection