從楊梅葉中提取黃酮的研究

化工論文編號:HG184  論文字數:10894,頁數:24

目  錄
中文摘要 I
英文摘要 II
目錄 III
1  緒論 1
1.1  前言 1
1.2  文獻綜述 1
1.2.1  黃酮類化合物的結構 1
1.2.2  楊梅葉中化學成分的研究 2
1.2.3  黃酮提取方法的研究 3
1.2.4  黃酮類化合物純化方法的研究 4
2  實驗部分 6
2.1  材料與儀器 6
2.1.1  材料與試劑 6
2.1.2  儀器 6
2.2  實驗方法 6
2.2.1  楊梅葉中總黃酮的提取 6
2.2.2  從楊梅葉中提取總黃酮工藝的優化 6
2.2.3  標準溶液的配制和標準曲線的制定 8
2.2.4  楊梅葉中提取物的純化 9
2.2.5  楊梅葉總黃酮含量測定 10
3  實驗結果與分析 11
3.1  單因素實驗 11
3.1.1  乙醇濃度對提取率的影響 11
3.1.2  料液比對提取率的影響 11
3.1.3  提取時間對提取率的影響 12
3.1.4  提取溫度對黃酮提取率的影響 13
3.2  正交試驗 13
3.3  楊梅葉中提取物的純化 15
4  結論 16
5  總結與展望 17
致謝 18
參考文獻 19

摘要：以粉碎的楊梅葉為材料，對總類黃酮提取條件和純化方法進行研究。采用有機溶劑浸提法，分別從乙醇濃度，料液比，提取時間，提取溫度等4個因素進行單因素實驗，并通過正交設計對楊梅葉黃酮提取條件進行了優化，確定了楊梅葉的最佳提取條件為50％乙醇，提取溫度為60℃，乙醇與楊梅葉20∶1，提取時間24h，提取率可達7.532％，顯著高于次佳工藝。并通過柱層析對萃取物進一步純化，得到純度為92. 32％的總類黃酮提取液浸膏。因此，該提取與純化方法對楊梅葉總類黃酮工業化生產具有一定的應用價值。
關鍵詞：楊梅葉；黃酮類化合物；提取

Abstract: The conditions for extracting and the method for purifying flavonoids from myrica rubra leaves were studied in this article. And the main factors which effect the distill rate were also discussed. The results from L9(34)table shows that the extracting temperature was 60℃, the ratio of 50％ ethanol to myrica rubra leaves was 20∶1, the time was 24 h. And the extractive ratio could be up to 7.532％, which was significantly higher than that under the Hypo - op timal conditions. And the extracts were further purified by silica gel column chromatography, the purity of the total flavonoids could be up to 92.32％. So the conditions above have some app lication value in industrial p rocessing of extracting total flavonoids from myrica rubra leaves.

Keywords: myrica rubra leaf; flavonoids compound; extraction