梓醇的提取方法的工藝研究

論文編號:HG293   論文字數(shù):18099,頁數(shù):28

摘要
 玄參科植物地黃(Radix Rehmanniae)是一種重要的常用傳統(tǒng)補益中藥,梓醇作為地黃中主要成分，屬于環(huán)烯醚萜苷類。文獻研究表明其對實驗性糖尿病有降血糖作用。鮮地黃中環(huán)烯醚萜苷類化合物結(jié)構(gòu)相似,極性較大,而且穩(wěn)定性差,較難分離。本課題以中草藥地黃為原料分別采用甲醇回流、大孔吸附樹脂吸附；甲醇溶解，超聲提取；乙醇梯度洗脫等方法提取，活性炭吸附，探討了地黃中梓醇的最佳提取工藝。采用高效液相色譜分析法進行鑒定，通過梓醇得率比較，得出如下結(jié)論：3倍量80%的乙醇,超聲提取45分鐘，提取液用大孔吸附樹脂D101吸附，用乙醇溶液梯度洗脫，濃縮，干燥，梓醇的收率為：0.32％。

關(guān)鍵詞：鮮地黃; 梓醇; 大孔吸附樹脂; 活性炭;  HPLC

 Abstract       Scrophulariaceae plant to Radix Rehmanniae is an important commonly used traditional Chinese medicine replenishing, catalpol as the main component in yellow, are iridoid glycosides category. Documents show that its research on experimental diabetes have hypoglycemic effect. Fresh Huang iridoid glycosides compounds structurally similar, more polar, but poor stability, the more difficult separation. This subject to Chinese herbal medicine and Huang were used as raw material methanol return macroporous resin adsorption; methanol dissolved, ultrasonic extraction; ethanol gradient elution methods such as extraction, activated carbon adsorption, and Huang discussed in extracting the best catalpol Process. A high performance liquid chromatography analysis to identify, through Catalpol yield, reached the following conclusions: three times the volume of 80 percent ethanol, ultrasonic extraction 45 minutes, extracts of using macroporous resin D101 adsorption, and Ethanol gradient elution, condensed, dry, Catalpol the yield: 0.32 percent.
  Key words: fresh yellow; Catalpol; macroporous resin; activated carbon and diatomite; HPLC;

 目錄
摘要 1
Abstract 2
1 文獻綜述 4
1.1地黃的主要成分及作用 4
1.1.1地黃的分類 4
1.1.2地黃的化學成分 5
1.1.3地黃的藥理學作用 6
1.2梓醇的化學結(jié)構(gòu)及作用 8
1.2.1梓醇的分布 8
1.2.2梓醇的化學結(jié)構(gòu) 8
1.2.3梓醇的藥理學作用 8
1.3梓醇目前的提取方法 9
1.4梓醇洗脫的原理 9
1.5 三種最佳萃取條件下的試驗方法 10
1.5.1甲醇回流提取法 10
1.5.2乙醇回流提取法 10
1.5.3乙醇超聲提取法 10
1.7梓醇的測定方法 11
1.7.1量差法測含量 11
1.7.2紅外法測定結(jié)構(gòu) 11
1.7.3高效液相色譜法測定含量 11
1.7.4薄層掃描法測定梓醇 12
1.8本論文的選題及意義 12
2實驗 14
2.1材料和儀器 14
2.2實驗部分 15
2.2.1地黃甲醇回流提取正交試驗 15
2.2.2地黃乙醇回流提取正交試驗 16
2.2.3地黃乙醇超聲提取正交試驗 18
2.2.4實驗原理 19
2.2.5三種試驗方法 20
2.2.6梓醇成品的精制 21
3 討論 22
 3.1梓醇提取率的測量 22
3.2紅外法鑒定梓醇結(jié)構(gòu) 22
3.3高效液相色譜法（HPLC） 23
3.4薄層掃描法測定梓醇 25
3.5對比試驗結(jié)果 25
4 總結(jié) 26
參考文獻 27
致    謝 28