豬源雙歧桿菌發酵工藝的研究

食品科學論文編號:SP035  論文字數:13304,頁數:31

 
 目  錄
 
摘  要 I
 ABSTRACT II
1引言 1
1.1 研究背景 1
 1.1.1 研究單位簡介..........................................1
1.2 雙歧桿菌的作用 2
 1.2.1 改善人體腸道功能......................................2
 1.2.2 恢復人體菌群平衡，形成抗菌生物屏障，維護人體健康......2
 1.2.3 控制人體內毒素的水平，保護肝臟病增強肝臟的解毒、排毒功能
 ............................................................2
 1.2.4 降血脂作用，防止動脈硬化個高血壓.......................2
 1.2.5 免疫調節作用...........................................3
 1.2.6 營養作用...............................................3
 1.2.7 提高SOD酶活力，消除人體自由基.........................3
 1.2.8 抗腫瘤、預防癌癥.......................................4
 1.3 研究意義.................................................4
 1.3.1雙歧桿菌開發現狀........................................4
 1.3.1.1 雙歧桿菌因子的開發與應用 ............................4
 1.3.1.2 雙歧桿菌營養液 ......................................4
 1.3.1.3 新型酸奶的開發與應用 ................................5
 1.3.1.4 雙歧桿菌藥品的開發...................................5
 1.3.2 雙歧桿菌生物工程展望...................................5
 2  材料..................................................7
 2.1樣品來源..................................................7
 2.2主要實驗試劑..............................................7
 2.3主要儀器與設備............................................7
 3 雙歧桿菌的分離和鑒定..................................8
 3.1實驗方法..................................................8
 3.1.1 分離純化方法...........................................8
 3.1.2 厭氧培養...............................................8
 3.1.3 仔豬腸道雙歧桿菌的初步鑒定.............................8
3.2 本章小結............................................9
4仔豬腸道雙歧桿菌的生長特性研究 10
4.1 操作方法 10
 4.1.1 試驗方法 10
4.1.2 檢測方法 10
 4.2 培養溫度 10
4.3 起始pH.............................................11
 4.4 培養基的選定............................................12
 4.4.1 碳源..................................................12
 4.4.2 氮源..................................................12
 4.4.3 生長因子..............................................12
 4.5測定雙歧桿菌的生長曲線............................13
 4.6 本章小結.............................................15
5  雙歧桿菌發酵工藝條件的優化研究........................16
 5.1 雙歧桿菌制劑的生產工藝流程..............................16
 5.2 發酵方案的確定..........................................16
 5.3 厭氧發酵培養基組成及發酵條件的確定......................16
 5.3.1 厭氧發酵的培養基組成..................................16
 5.3.2 發酵控制條件..........................................16
 5.4 發酵液活菌數的檢測......................................16
 5.5 10L發酵罐擴大培養試驗..................................17
 5.6 100L發酵罐擴大培養試驗................................18
 5.7 本章小結...............................................18
 6  結論與展望...............................................19
 6.1 結論....................................................19
6.2 展望...............................................19
參考文獻 20
附錄I 培養基的配置 21
 附錄II  雙歧桿菌的糖代謝途徑...........................22
謝辭 24

摘  要

 雙歧桿菌是人體內存在的一種生理性細菌，是人體有益菌中最值得重視和研究的一種，它與人體的健康密不可分，可以說是大自然賜予人類的健康法寶。雙歧桿菌的研究和生產具有很好的發展前景。
 雙歧桿菌一般能從動物身上提取，豬的胃上皮細胞黏膜附有有大量雙歧桿菌。從豬的糞便中分離出雙歧桿菌，并將其擴大生產。合適的生長條件對雙歧桿菌的生長速度、菌體形態、活菌數、代謝產物有良性的影響。因此通過研究，獲得雙歧桿菌生產的最優工藝方案有非常重要的意義。

關鍵詞：雙歧桿菌  發酵工藝 
 
 
 THE STUDIES OF PIGGED-SOURSE BIFODOBACTERIUM FERMENTATION PROCESS
ABSTRACT
 
 Bifodobacterium is a sort of physiological bacteria inside human body, and is also one of the probiotics which deserves to be valued and researched on. It is inseparably connected with human health and could be described as a treasure of heath given to humanity by the nature. Therefore, the research and production of bifodobacterium could prove to be promising.
 Bifodobacterium is partly extracted from an animal, like a pig, who has a huge amount of epith cells of gastric mucosa. Thus bifodobacterium could be isolated from the excrement of pigs and the cultures could be expanded. A proper condition of culture has a positive effect on the growth speed, morphological characteristics, number of vial bacteria and metabolites of bifodobacterium. Therefore this research is crucially important to acquire an optimal technology scheme of the culture of bifodobacterium.

Key Words: bifodobacterium  fermentation process