海帶中褐藻糖膠提取工藝研究

食品科學論文編號:SP041  論文字數:9223,頁數:21  附任務書,開題報告

目  錄
1. 前  言 4
1.1 選題背景 4
1.2 褐藻糖膠的綜述 4
1.2.1  褐藻概況 4
1.2.2  褐藻糖膠概況 4
1.2.3  褐藻糖膠的生物活性功能 5
1.2.4  褐藻糖膠國內外研究現狀 6
1.3  研究方法 8
1.4  研究目的和意義 8
1.5  預期結果 8
2.材料與實驗方法 9
2.1  材料與儀器 9
2.1.1  原料與試劑 9
2.1.2  儀器 9
2.2  實驗方法 9
2.2.1  褐藻糖膠的提取 9
2.2.2  褐藻糖膠的純化 10
2.2.3  褐藻糖膠的測定 10
2.3  不同實驗條件對海帶中褐藻糖膠提取影響 10
2.3.1  pH單因素實驗 10
2.3.2  溫度單因素實驗 10
2.3.3  時間單因素實驗 11
2.3.4  液料比單因素實驗 11
3. 結果與分析 12
3.1  pH單因素實驗結果與分析 12
3.2  溫度單因素實驗結果與分析 13
3.3  時間單因素實驗結果與分析 14
3.4  液料比單因素實驗結果與分析 15
3.5  結論 16
4. 褐藻糖膠的研究前景 17
參考文獻 18

摘  要：海帶含有多種生物活性成分，如海帶酸、褐藻酸、甘露醇和褐藻糖膠等，其中褐藻糖膠具有降血脂、抗血凝、抑制腫瘤細胞生長、調節免疫力、抗輻射等作用。鑒于褐藻糖膠的諸多保健功能和我國有豐富的海帶資源，開展對海帶資源的深加工和綜合利用、充分發揮和利用褐藻糖膠這以天然多糖資源，對提高海帶附加值、發展我國海帶資源加工有深遠的意義。本文采用酸提法從海帶中提取褐藻糖膠，采用乙醇分級沉淀法進行純化。主要探討了提取時間、溫度、pH、料液比等因素對提取率的影響。結果表明，在提取溫度70 ℃，pH2.0，液料比為30，提取4h時，提取率最高，達到7.3%。
關鍵詞：海帶；褐藻糖膠；酸提法；沉淀法

Abstract: The kelp contains a variety of biological active ingredients, such as kelp acid,brown alga acid,mannitol and fucoidan,among which fucoidan has hypolipidemic effect on anti-blood congealing,inhibits the growth of tumor cells to regulate immune function,anti-radiation effects.
In view of the fact that fucoidan has many health functions,and China has abundant seaweed re - sources,therefore,the development to the kelp resources,intensive processing and the comprehen- sive utilization,the full display and the use fucoidan which is natural polysaccharide resource,to enhance the kelp added value and to develop our country kelp resources processing has the pro- found significance.This paper uses the sour formulation to extract the fucoidan from the kelp,uses the ethyl alcohol graduation precipitation method to carry on the purification. Focuses on the extr- action time,the temperature,pH,solid to liquid ratio on the extraction rate. The result indicated that in the extraction temperature 70℃,pH 2.0,70% ethyl alcohol density,extraction 4 h,the extraction rate is 7.3%.
Key words: kelp; fucoidan;acid distill;precipitation