杜仲生物活性物質提取的工藝研究

論文編號:SW227  論文字數:15269,頁數:26

摘要：本文提取的杜仲生物活性物質有黃酮、綠原酸、桃葉珊瑚甙等。本論文通過對回流提取、超聲波提取、微波提取和半仿生提取等杜仲生物活性物質提取工藝進行比較研究，確定了較佳的提取工藝為超聲波輔助乙醇回流提取法。通過探索試驗和對照試驗確定了超聲波輔助乙醇回流提取法的基本工藝參數范圍，然后采用正交試驗方法，確定了超聲波輔助乙醇回流提取法提取杜仲葉中生物活性物質的優化工藝參數為：采用料液比為1：12，濃度為60%的乙醇溶液，在75℃高頻率下超聲預處理30min后， 85℃下回流提取40min，01提取2次，濃縮干燥得到產物，其干浸膏提取率可以達到20.95%，總黃酮類物質和綠原酸總提取率可以達到3.81%。
關鍵詞： 超聲波提取；杜仲；總黃酮類物質；綠原酸

Abstract: In this article, some bioactive components such as flavone、Chlorogenic Acid、Aucubin are extracted from the Eucommia ulmoides by refluence extraction method、Ultrasonic-assisted extraction method and microwave-assisted extraction method. The results show that the ethanol refluence extraction with Ultrasonic wave assisted method is the best way. Upon the comparative experiments and the orthogonal test, the optimum extraction conditions are: the proportion of the Eucommia ulmoides and ethanol is 1:12, the concentration of the ethanol is 60%, pre-process sample 30 min in 75℃ with high frequency of Ultrasonic wave method. Then we extract the bioactive components 40 min at the active temperature 85℃ with refluence extract method. Repeat the process again. The total extraction yield of active compounds is as high as 20.95%, and the total extraction yield of the flavone and Chlorogenic Acid is as high as 3.81%.
Key words：Ultrasonic extraction；Eucommia ulmoides；Flavonoid compound；Chlorogenic Acid