三孢布拉氏霉菌發酵生產β-胡蘿卜素的條件研究

論文編號:SW228  論文字數:12763,頁數:24

摘  要：本文以三孢布拉氏霉菌正、負菌為生產菌種，篩選了產孢子培養基及優化了β-胡蘿卜素的生物合成條件。其最佳的產孢子培養基：麥芽汁雙層平板，在此培養基中，菌絲生長茂盛，產孢子多；通過對不同碳、氮源的組合篩選，確定最佳的碳、氮源及發酵終止時間，其結果為：淀粉作為碳源，黃豆粉作為氮源，發酵144 h，β-胡蘿卜素合成量最大。在此基礎上，利用Plackett-Burman設計分析了發酵培養基中各成分對β-胡蘿卜素產量的影響，其結果為：對β-胡蘿卜素產量影響的重要因素為β-紫羅酮，VB1，淀粉，植物油。最后，對該4因素以L9(34) 正交設計進一步優化，得出最佳的培養基組成為：淀粉41 g/L，黃豆粉20 g/L，植物油35 ml/L，磷酸二氫鉀2 g/L，硫酸鎂1 g/L，VB1 0.030 g/L，發酵40~44 h后，添加0.05%的異煙肼12 ml/L及10%的β-紫羅酮15 ml/L。β-胡蘿卜素的發酵產量較比優化前發酵培養基提高了16.78%。
關鍵詞：三孢布拉氏霉菌；β-胡蘿卜素；培養基；Plackett-Burman設計；正交設計

Abstract：The produce industrially of β-carotene by Blakeslea trispora (+ −) was studied, and the process of β-carotene synthesis was optimized. The best spore produced medium was filtrated: the double layer wort plate, the mycelial growed flourishing, and produced much more spores. The fermentation medium of synthesizing β-carotene with Blakeslea trispora was optimized. The optimum carbon source, nitrogen source and the fermentation time was obtained through the compounding of different carbon source and nitrogen source. The results showed that faecula was used as carbon source, bean flour was used as nitrogen source, fermentation time was 144 h. Base on this result, the important factors which affect fermentation conditions of β-carotene were screened by Plackett-Burman design. Then by using orthogonal test, these important factors were optimized. Results showed that the best fermentation medium were faecula, 41 g/L; bean flour, 20 g/L; Vegetable oil, 35 ml/L; KH2PO4, 2 g/L; MgSO4, 1 g/L; VB1, 0.030 g/L. Isoniazid and β-ionone was appended after fermenting 40~44 h. Its yield of β-carotene increase 16.78% compared with the original fermentation medium.
Keywords: Blakeslea trispora; β-carotene; Culture medium; Plackett-Burman design;Orthogonal design