艾葉中總黃酮的提取及其抗氧化活性研究

論文編號:SW226  論文字數:9010,頁數:18

摘要：對從艾葉中提黃酮類化合物的工藝進行了研究，在提取過程中，通過單因素實驗分析采用NaNO2-Al(NO3)3-NaOH顯色法,對提取液濃度、提取溫度、提取時間及料液比等4個主要因素對提取率的影響，在單因素的基礎上，通過正交實驗，得到最佳提取工藝, 乙醇濃度為40%，料液比為1：40，提取溫度為70℃，提取時間為3h，其中最主要的影響因素是提取溫度。再利用“還原活性”“總抗氧化活性”、“超氧陰離子”等抗氧化模型，對從中提取得到的總黃酮的抗氧化活性進行考察。
關鍵詞：艾葉;黃酮類物質;抗氧化;提取

Abstract: Total flavonoids were extracted from Folium Artemisiae Argyi. The extraction rate of flavonoids was detected by adoption NaNO2-Al(NO3)3-NaOH color analysis. On the basis of a single factor, the extraction rate was measured through orthogonal experiment in this article. The best extraction conditions were concentration of 40% ethanol, feed ratio of 1: 40 and temperature at 70℃ for 3 hours. Reducing ability, total antioxidant activity and superoxide anion radical scavenging assays were carried out to eva luate the antioxidant potential of the total flavonoids.
Key words: Folium Artermisiae Argyi; Flavonoids; Antioxidant; Extraction