培養條件對枯草芽孢桿菌黑色變種芽孢形成的影響

生物工程論文編號:SW051  論文字數:9612,頁數:22

目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1 枯草芽孢桿菌概況 1
 1.2 本項目的研究目的及意義 5 
2. 實驗部分 7
 2.1 材料與方法 7
 2.2 試驗設計 9
3. 結果與討論 10
 3.1 ATCC 9372生長曲線的測定 10
 3.2 最佳培養基組成試驗結果 11
 3.3 培養條件單因素試驗結果 11
 3.4 小結 15
4．總結與展望 16
致謝 17
參考文獻 18

摘要：為了提高枯草芽孢桿菌黑色變種ATCC 9372的芽孢形成率，在最佳培養基確定的基礎上，研究培養條件，包括初始pH、培養溫度、接種量和溶氧水平，對芽孢形成率的影響。試驗結果表明，最佳的培養條件為培養基（葡萄糖5g，蛋白胨5g，NaCl5g，MnSO4·H2O0.04g），于pH7.0，接種量5%，裝瓶量為25ml/250 ml錐形瓶，30C搖床培養24小時，此時，芽孢數量最多可達到3.4×107cfu/ml，芽孢形成率達到94.4％。
關鍵詞：枯草芽孢桿菌黑色變種 ；生長曲線 ；培養條件；芽孢形成

Culture Conditions Affecting Spore Production of Bacillus subtilis var. niger

Abstract:  In order to enhance spore production of Bacillus subtilis var. niger ATCC 9372, on the basis of optimal medium, culture conditions, including initial pH、temperature and dissolved oxygen levels on its spore production were examined. Results suggested that the optimal medium composition for spore production was as follows lactose 5g, peptone 5g, NaC1 5g, MnS04·H20 0.4 g, H20 1000 ml, optimal initial pH was 7. 0 and appropriate medium volume was 25 ml in 250 ml flask. The number of spores could reach 3.4 x 107 cfu/m1, and the highest spore formation was 94.4％ at 30℃, 150 r/min for 24 h.
Key words：Bacillus subtilis var. niger; growth carve; culture conditions; spore production