枯草芽孢桿菌的優化培養（液態發酵）

生物工程論文編號:SW065  論文字數:11836,頁數:34

目   錄

中文摘要……………………...…………………………………………………………..I
英文摘要……………………………………………………………………………....…II
 目    錄………………………………………………………………...………….…..III
1.  緒論……………….…………………………………………………………………..1
 1.1  枯草芽孢桿菌極其微生態制劑介紹………………………………………....1
 1.2  枯草芽孢桿菌微生態制劑應用及前景……………………………..………..1
2.  實驗部分………………………….……….………………………………….….......3
 2.1  材料……………………………………………………………………………3
 2.2  實驗方法……………………………………………………………………...3
 2.3 微生物的生理化反應…………………………………………………………..4
 2.4 小結………………………………………………………………………12
3.  結果與討論…………………………….…………………………………………..14
 3.1培養基優化…………………………………………………………………….14
 3.2培養條件優化………………………………………………………………….15
 3.3 最適發酵條件驗證…………………………………………………………....19
 3.4 生理生化特性檢測結果………………………………………………………21
    3.5實驗結論…..…………………………………….…….……………………….22
4.總結與展望……………………………………………………………...………….....24
致謝……………………………………………….………………………..……............26
參考文獻……………………………………………………………….….…………….27
附錄A  各因素對生長量影響的數據表………………………………………………30

摘 要：通過單因素實驗和正交實驗，研究了枯草芽孢桿菌(Bacillus subtilis)的液體發酵培養基和工藝條件，并對原始菌種發酵培養基的碳源、氮源等的選擇及配比進行調整，以確定其各項要素的合理配比。確定最佳的培養基為：蔗糖3% ，蛋白胨1% ，酵母膏0.5% ，KH2PO4 0.45%，(NH4)2SO4  0.25% ，CaCO3 0.2% ；并對培養基單一發酵條件的改變，通過培養結果得出該條件下的最佳值，應用于具體實驗操作中去，最終獲得溫度、pH值、裝量和接種量四項發酵因素下的最佳組合，并對該組合進行驗證，證明該最佳組合是否能真正達到提高菌種發酵培養的目的。實驗證明最適發酵條件為：溫度為30%，pH值為7.0，裝量為20ml/250ml錐形瓶，接種量為5%，轉速200r/min條件下。

關鍵詞：枯草芽孢桿菌(Bacillus subtilis)；培養基；發酵條件；生長量。

Bacillus subtilis Optimization Training  (liquid fermentation)

Abstract : The single factor and orthogonal experiment. of Bacillus subtilis (Bacillus subtilis) and the liquid fermentation medium conditions. primal bacterium medium carbon, nitrogen ratio and the choice of adjustment to determine the elements of a reasonable ratio. Determine the best medium : 3% sucrose, 1% peptone, yeast extract 0.5% KH2PO4 0.45% (NH4) 2SO4 0.25%, 0.2% CaCO3; also a single medium fermentation conditions change, the results of the conditions through the cultivation of the best values application specific experimental operation, and eventually the temperature, pH value, inoculum load and the best combination of four factors fermentation, and this combination of certification, proved the best mix of bacterium can truly improve training purposes. Experimental evidence for the optimum conditions : temperature 30%, pH 7.0, 20ml/250ml installed capacity of tapered bottles inoculation of 5% speed agitation conditions.

Keywords：Bacillus subtilis； Medium ；Fermentation conditions；Growth.