羊肚菌深層發酵和多糖提取分析

生物工程論文編號:SW098  論文字數:10174,頁數:30

目錄
中文摘要 Ⅰ
英文摘要 Ⅱ
1.緒論 1
 1.1羊肚菌概述 1
 1.2多糖的分離方法簡介 2
 1.3 多糖的測定 4
 1.4 羊肚菌發酵的影響因素 5
 1.5研究現狀分析與項目意義 6
2．實驗部分 7
 2.1  實驗材料 7
 2.2  實驗方法 8
3.結果與討論 13
 3.1葡萄糖標準曲線 13
 3.2發酵條件的確定 13
 3.3氨基酸的檢測 15
 3.4單糖的檢測 16
4.總結與展望 21
 4.1實驗總結 21
 4.2展望 22
致  謝 23
參考文獻 24

摘要：羊肚菌是一種十分重要的食藥兼用真菌，含有豐富的生理活性物質，多糖是其中的重要一種，具有增強肌體免疫力、抗疲勞、抗病毒、抑制腫瘤等多種功能。本實驗羊肚菌液體發酵最適發酵溫度為25℃，最適的轉速為150rpm；溶解氧會抑制菌絲體的生物量和多糖和積累；分離菌絲體和發酵液，然后采用超聲破壁，1:40的浸提比浸提3h，浸提次數為兩次，用95%的乙醇醇析2d，再用無水乙醇洗滌兩次、Sevage法脫蛋白后用ΦMW3500 透析制取胞內和胞外多糖；采用苯酚-硫酸法測定胞內多糖的總糖，最高的得率有3.5%；液相分析結果表明，胞內還有游離的單糖，胞外則不含游離的單糖；用2mol/L的硫酸水解后的多糖進行液相分析，結果表明，羊肚菌多糖可能有葡萄糖、半乳糖、甘露糖、木糖。
關鍵字：羊肚菌；發酵；多糖；單糖；液相色譜法

Deep fermentation fo Morchella and extraction of polysaccharide

Abstract: Morchella is a very important edible and medicinal fungi, is rich in physiologically active substances, polysaccharide is one of the important one, with enhanced body immunity, anti-fatigue, anti-virus, and many other features of tumor. In this experiment, the optimum fermentation fermented morel liquid temperature was 25 ℃, the optimal speed is 150rpm; Dissolved oxygen will inhibit the mycelium biomass and polysaccharide and accumulation; Separation of mycelium and fermentation broth, and then broken using ultrasonic, 1:40 extraction of ratio, extraction 3h, extraction times for the two, with 95% ethanol precipitation 2d, then using ethanol washing twice and suing  Sevage  removal protein , dialysis with ΦMW3500 ,the and Receive the polysaccharide. Liquid chromatography analysis showed that there are free intracellular monosaccharide, is non-free extracellular monosaccharide; With 2mol / L sulfuric acid hydrolysis of the polysaccharide Liquid chromatography analysis, the results show that the polysaccharides may be glucose, galactose, mannose, xylose.
Keywords: Morchella; Fermentation; Polysaccharide; Single sugar; Liquid chromatography