固定化細(xì)胞法生產(chǎn)醋酸的優(yōu)化研究

生物工程論文編號:SW112  論文字?jǐn)?shù):14869,頁數(shù):27

中文摘要┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉Ⅰ
英文摘要┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉Ⅱ
目錄┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉Ⅲ
1.緒論┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉1
 1.1 除冰劑的使用概況┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉1
 1.2 用海藻酸鈉固定化醋酸菌┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉4
 1.3 固定化細(xì)胞的特性┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉4
 1.4 醋酸菌的醋酸發(fā)酵原理┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉5
 1.5發(fā)酵培養(yǎng)中醋酸細(xì)胞的生長狀況 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉6
2．材料和方法 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉7
 2.1材料 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉7
 2.2 實驗方法與制備過程 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉8
 2.3 分析步驟 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉8
3. 結(jié)果和討論  ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉10
 3.1 接種環(huán)數(shù)優(yōu)化 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉10
 3.2 葡萄糖標(biāo)準(zhǔn)曲線制作 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉12
 3.3 菌種OD值相關(guān)性研究┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉13
 3.4 酒精初始濃度對乙酸產(chǎn)量的影響研究 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉14
 3.5酒精初始濃度對菌體個數(shù)的影響研究┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉16
 3.6酒精初始濃度對葡萄糖消耗的影響研究┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉17
 3.7 酒精初始濃度對乙醇消耗的影響研究 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉19
 3.8 小結(jié) ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉20
4. 總結(jié)┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉21
致謝 ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉22
參考文獻(xiàn) ┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉┉23

摘  要：本文是以海藻酸鈉為載體，固定化醋酸菌發(fā)酵乙醇生產(chǎn)醋酸。研究了固定化細(xì)胞的制備，制備的顆粒的半徑和體積大小。另外，還對接種環(huán)數(shù)、種子培養(yǎng)時間進(jìn)行了優(yōu)化。最主要的是研究了不同酒精初始濃度對乙酸產(chǎn)量、菌體個數(shù)、菌體生長周期、葡萄糖消耗量、乙醇消耗量的影響。最后我們得到如下結(jié)論:（1）固定化細(xì)胞顆粒平均直徑為5.0mm，顆粒體積為65.42mm3；（2）優(yōu)化接種數(shù)在15環(huán)、培養(yǎng)時間為36-48小時之間，發(fā)酵液菌體個數(shù)最多；（3）葡萄糖濃度與葡萄糖OD值的換算公式：y=4.7237x，單位為mg/ml；（4）菌體個數(shù)與菌液OD值的相關(guān)性：y = 402.88x，單位為107個/毫升；（5）酒精初始濃度對乙酸產(chǎn)量的影響：在各酒精濃度中，5%酒精濃度的發(fā)酵液乙酸轉(zhuǎn)化率最高，最佳培養(yǎng)時間為96小時；（6）酒精初始濃度對菌體個數(shù)的影響：不同的酒精初始濃度影響著菌體的生長，酒精濃度越高對菌體的生長起著延長生長延遲期的作用；（7）酒精初始濃度對葡萄糖消耗的影響：乙醇的含量越高則葡萄糖的消耗就越少；（8）酒精初始濃度對乙醇消耗的影響：在72小時時間點，乙醇消耗完畢后，醋酸在72-96小時時間段的繼續(xù)生成，是由于乙醛沒隨乙醇的消耗完畢馬上轉(zhuǎn)化為醋酸而引起的滯后反應(yīng)。

關(guān)鍵詞：固定化；乙醇；醋酸菌；醋酸鈣鎂鹽
 
Immobilized Cell Production Of Acetic Acid Bacteria Optimization

Abstract: This paper is based on alginate as a carrier of fixed acetic acid fermentation ethanol production of acetic acid. Study of the immobilized cells Preparation, preparation of particle radius and the size. Also, the ring vaccination, seed cultivation time for the optimization. The most important thing is the alcohol of different initial concentrations of acid production, cell number, cell growth cycle, glucose consumption, the effects of ethanol consumption. Finally, we get the following conclusions : (1) a fixed cell average particle diameter of 5.0mm, particle size of 65.42mm3; (2) optimize the number of vaccines in 15 Central, Training time for between 36-48 hours, the number of bacteria broth up; (3) The concentration of glucose and glucose OD value of the conversion formula : y = 4.7237x, unit of mg/ml; (4) the number of bacteria cells OD value of the correlation : y = 402.88x, 107 units/ml; (5) the initial alcohol concentration of acetic acid production : in the alcohol concentration. 5% alcohol concentration of acetic acid fermentation broth highest conversion rate, the best time to train 96 hours; (6) the initial alcohol concentration of cell number : The impact of different initial concentrations of alcohol affects cell growth, The higher the alcohol concentration in the cell growth plays an extended period of growth delay; (7) the initial alcohol concentration of glucose consumption : The impact of the higher ethanol content of the glucose consumption is smaller; (8) the initial alcohol concentration of ethanol consumption : The impact of the 72-hour time point, ethanol consumption has been completed, acetate in 72 -96 hours of the continued generation, Acetaldehyde is not with the consumption of alcohol finished immediately into acetic acid resulting from the lagging response
 Keywords：Immobilized；Ethanol；Acetic acid bacteria；Calcium magnesium acetate