米多霉素胞嘧啶衍生物的分離純化研究

生物工程論文編號:SW111  論文字數:15956,頁數:34

目錄
1 緒論 4
 1.1 前言 4
 1.2 抗生素分離純化的研究概況 5
 1.3 米多霉素類化合物的性質與作用 9
2 實驗部分 11
 2.1 實驗材料 11
 2.2 實驗內容與方法 14
3 結果與討論 20
 3.1 離子交換樹脂的靜態篩選 20
 3.2 pH值對DK110樹脂吸附容量的影響 22
 3.3 離子交換等溫線 23
 3.4 離子交換樹脂穿透曲線的測定 25
 3.5 洗脫工藝 26
 3.6 脫色工藝 29
4 總結與展望 32
致 謝 33
參考文獻 34

摘要：新型核苷類農用抗生素米多霉素胞嘧啶衍生物（Mildiomycin cytosine analogue，MIL-C）對各種植物的粉狀霉菌有強烈的拮抗作用，并且毒性較低。本文主要探索了用離子交換法從發酵液中分離MIL-C的工藝。通過靜態篩選，篩選到對MIL-C具有較大靜態交換容量和良好吸附過程動力學性能的大孔弱酸性陽離子交換樹脂DK110。實驗過程考察了不同pH、溫度、上樣濃度以及上樣速度對DK110吸附過程的影響。實驗結果表明溫度和上樣濃度對DK110吸附過程影響不大，實驗操作溫度可為室溫。上樣發酵液的pH和流速分別控制在7.0和2BV/h。同時也對洗脫條件進行了優化，確定最佳操作條件為：洗脫流速2BV/h，洗脫劑為2%氨水。另外對MIL-C發酵液的脫色工藝進行了考察，結果表明可選用陰離子樹脂D261在pH7.0進行脫色，接觸時間約為120min。該脫色工藝的回收率達到了84.71%。
關鍵詞：米多霉素胞嘧啶衍生物；離子交換法；DK110樹脂

Abstract: Mildiomycin cytosine analogue (MIL-C), a new nucleoside antibiotic, showed remarkable biological activity against powdery mildews of many plants with low toxicity. This dissertation will pay attention to the process of separating MIL-C from fermentation broth. MIL-C is expected to be separated by ion exchange resins. A weakly cationic exchange resin DK110 was selected from 8 different resins, which showed high static exchange capability and good dynamic character. The effect of pH, temperature, inlet concentration, flow rate to the process of adsorption was studied in this experiment. The temperature for adsorption process was selected as 25℃ because the exchange capability is not affected by the temperament and the inlet concentration. The optimized adsorption was obtained at pH 7.0, flow rate 2BV/h. The elution conditions were determined as follows: 2% NH3·H20 solution, elution flow rate 2BV/h. An effective technology to decolorize the fermentation broth was investigated in this thesis. D261 resin, a kind of anionic exchange resin, was selected and the discoloring conditions were optimized as follows: pH7.0, discoloring time 120 min. Under these conditions, the recovery efficiency achieved 84.71%.
 Key words: Mildiomycin cytosine analogue; Ion exchange; DK110 resin