紅曲霉生產紅色素菌株發酵條件的優

生物工程論文編號:SW143  論文字數:11355,頁數:30             
目  錄
中文摘要..........................................................I
英文摘要..........................................................II
目錄..............................................................III
1. 緒論............................................................1
1.1  紅曲色素具有較高市場應用價值...............................1
1.2  紅曲可色素以應用于各種食品中...............................1
 1.3  紅曲色素的組成及生成機制..................................2
 1.4  紅曲色素的生產方法........................................4
2. 實驗部分........................................................5
 2.1  材料與方法................................................5
3. 結果與討論......................................................7
 3.1  紅曲色素培養條件的優化....................................7
 3.2 紅曲色素的提取..............................................14
4．總結與展望.......................................................21
致謝...............................................................22
參考文獻...........................................................23

 摘 要：本論文是用產紅色素的紅曲霉菌株進行培養基及培養條件的優化，研究了幾種常見碳源，氮源，不同培養基，溫度，pH，不同量的大米粉對紅曲霉產紅色素及黃色素的影響。結果表明，碳源玉米粉，大米粉都有利于紅曲霉菌株產紅色素和黃色素。以碳源為玉米粉4%，大米粉4%產色素最多，以氮源為硝酸鈉，亞硝酸鈉為0.15%產色素最多。大米粉的含量在4%-5%之間的培養基產的色素最多。培養基以查式培養基最好，培養溫度32℃，pH6.0其產的色素最多。在通過單因素試驗研究提取次數，提取劑濃度、用量，浸提溫度，浸提時間對紅曲色素提取影響的基礎上，采用正交試驗研究了提取劑濃度、浸提時間、浸提次數對紅曲色素提取的影響。試驗結果表明，從干紅曲霉菌絲體中提取紅色素的最佳工藝條件為：以90％乙醇為提取劑，提取溫度40℃，提取時間40 min，在此條件下提取3次可使菌絲體中紅色素的提取率最高。

關鍵詞：紅曲霉；紅色素；色價；碳源；氮源
                 
                                  
Monascus red pigment production optimization of fermentation conditions

Abstract:
This paper is producing strains of Monascus red pigment media and culture conditions for optimization of several common carbon sources, nitrogen sources, different media, temperature, pH, different amounts of red rice flour, red pigment production by Aspergillusand the impact of yellow pigment.The results showed that carbon source corn flour, rice flour are conducive to producing strains of Monascus red pigment and yellow pigment.To carbon source for the corn starch 4%, 4% rice flour produced the most pigment, with nitrogen as sodium nitrate, sodium nitrite 0.15% maximum pigment production.Content of rice flour 4% -5% in the medium between the maximum pigment production.To investigate the best medium-type medium, temperature 32 ℃, pH6.0 most of its production of pigment..Single-factor test in the study by extracting times concentration, dosage, extraction temperature, extraction time effects on the extraction of red pigment based on the orthogonal test of the extract concentration, extraction time, extraction times on theextraction of red pigment.The results show that the mycelium of Aspergillus from the red pigment extracted the optimum conditions were: 90% ethanol as extraction solvent, extraction temperature 40 ℃, extraction time 40 min, under these conditions can bacteria extracted 3 timesfilaments in the maximum extraction rate of red pigment.
Keywords：Monascus; red pigment; color value; carbon; nitrogen