聚蘋果酸生產(chǎn)菌的篩選

生物工程論文編號(hào):SW144  論文字?jǐn)?shù):6966,頁數(shù):20

 目  錄
中文摘要 I
英文摘要 II
目錄 III
緒論 1
   1.1   聚蘋果酸簡(jiǎn)介 1
   1.2  聚蘋果酸應(yīng)用及前景 1 
   1.3  發(fā)酵法生產(chǎn)聚蘋果酸..........................................2
   1.4  課題目的....................................................3 
   1.5  課題內(nèi)容概述...............................................3 
實(shí)驗(yàn)部分........................................................3
   2.1 實(shí)驗(yàn)材料與方法.............................................3
   2.2.接種以及篩選菌種............................................5
   2.3菌落的鑒定和產(chǎn)物測(cè)定.........................................5
3. 結(jié)果與討論....................................................5
   3.1 菌種采集..................................................5
   3.2 菌種篩選..................................................5
   3.3 菌落鑒定結(jié)果與產(chǎn)物的測(cè)定..................................7
4．總結(jié)與展望....................................................14
致謝.............................................................15
參考文獻(xiàn).........................................................16

摘 要：本論文展示了聚蘋果酸性能特點(diǎn)以及在各種領(lǐng)域中的應(yīng)用前景。人工合成聚蘋果酸步驟復(fù)雜，成本高而轉(zhuǎn)向生物發(fā)酵生產(chǎn)聚蘋果酸。設(shè)立實(shí)驗(yàn)方案從自然界中篩選聚蘋果酸產(chǎn)生菌，提取大量的樣品通過琥珀酸培養(yǎng)基、L-蘋果酸培養(yǎng)基等篩選性平板的選育，通過形態(tài)的對(duì)比觀察，選出形態(tài)與目的菌種相似的菌落。經(jīng)過發(fā)酵培養(yǎng)后，用紙層析與液相色譜鑒定發(fā)酵產(chǎn)物是否有聚蘋果酸以及產(chǎn)量。

關(guān)鍵詞：生物高分子; 聚蘋果酸;生物發(fā)酵；出芽短梗霉；紙層析
Poly malic acid producing bacteria screening

Abstract: This paper demonstrates the characteristics of polyethylene, and PMA can be in various fields of application.Artificial chemical synthesis of poly malic acid step is complicated and costly,sowe turn to the production of bio-fermentation malic acid.Set up screening programs in screening poly malic acid producing bacteria from the natural world.Extraction of a large number of samples.Through the succinate medium and L-malic acid medium al training.Through the comparison of observed patterns,selected with similar bacteria colony.After fermentation,identification of fermentation products with Paper chromatography and liquid chromatography to determine whether it contains malic acid.
Keywords: Biopolymer; poly malic acid; bio-fermentation; Aureobasidium pullulans; paper chromatography