竹葉活性物的提取及抑菌效果研究

生物工程論文編號:SW154  論文字數:9844,頁數:22

目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1  研究背景和意義 1
 1.2  竹葉的國內外現狀 1 
2. 實驗部分 2
 2.1  實驗材料和儀器 2
  
 2.2  實驗方法 3
3. 結果與討論 6
 3.1  不同提取方法提取竹葉有效成分的抑菌試驗 6
 3.2 不同提取方法提取竹葉有效成分的含量測定 9
4．總結與展望 13
致謝 15
參考文獻 16
附錄 18

摘 要：本論文研究了竹葉活性成分的提取工藝及其抑菌效果。采用濾紙片法、稀釋平板計數法測定毛竹不同提取方法和溶劑多級萃取后萃取物對3種不同供試菌的抑菌作用。結果表明：竹葉提取物對金黃色葡萄球菌、枯草芽孢桿菌抑菌效果較好，對大腸桿菌抑菌效果不明顯；且溫度80 ℃、乙醇體積分數60 %、料液比1：8、醇提1 h，石油醚萃取后正丁醇萃取水層提取物的抑菌效果最好；對金黃色葡萄球菌的抑菌圈大小為34 mm，對枯草芽孢桿菌的抑菌圈大小為32 mm；
關鍵詞：竹葉；提取；抑菌

Extraction of Active Components from bamboo leaves and their anti-microbial actives

Abstract: In this article, the extraction technology of active components from bamboo leaves and their anti-microbial actives were studied. The anti-microbial activity of different extracts with different extraction methods and solvent extraction was defermined by filter paper method and dilution plate count method. The results showed that the extracts of bamboo leaves have better anti-microbial activities against Staphylococcus aureus and Bacillus subtills and have poor anti-microbial activities against Escherichia Coli. In all extracts, the water extract after the petroleum ether and butanol extraction had the best anti-microbial activities with the extract condition: 60℃, 60 %（v/v）ethanol, solid/liquid ratio 1:8, 1h, the mean diameters of Staphylococcus aureus was 34mm, and the mean diameters of Bacillus subtills was 32mm, espectively.
Keywords：Bamboo leaves;  Extract;  Anti-microbial