產(chǎn)紅曲色素的紅曲霉菌種篩選及發(fā)酵條件優(yōu)化

生物工程論文編號:SW153  論文字數(shù):10021,頁數(shù):22

 Ⅳ
目  錄
中文摘要 Ⅰ
英文摘要 Ⅲ
目錄 Ⅴ
1. 緒論 1
 1.1  紅曲及紅曲霉簡介 1
 1.2紅曲霉分離原理的研究及培養(yǎng)條件的設(shè)計 2 
 1.3紅曲霉的生物學(xué)特征 2
2. 實驗部分 3
 2.1主要實驗儀器、設(shè)備 3
 2.2主要實驗試劑 3
 2.3培養(yǎng)基 3
 2.4實驗方法與步驟 4
 2.5紅曲霉液體培養(yǎng)條件與產(chǎn)色素關(guān)系的研究 5
3. 結(jié)果與討論 6
 3.1紅曲霉的分離純化結(jié)果與分析 6
 3.2紅曲霉液體培養(yǎng)條件的單因素實驗結(jié)果與分析 9
 3.3正交試驗最佳發(fā)酵培養(yǎng)條件的結(jié)果與分析 11       
4．總結(jié)與展望 12
 4.1總結(jié) 12
 4.2展望 13
致謝 15
參考文獻 16

摘要：根據(jù)紅曲霉菌的性質(zhì)，本文采用無菌過濾法收集經(jīng)研磨后的紅曲米中的紅曲霉孢子懸液，運用平板稀釋法，實現(xiàn)了從紅曲米中簡易分離紅曲霉。根據(jù)紅曲霉的生物學(xué)特征，總結(jié)出分離紅曲霉的原理以及分離培養(yǎng)基的設(shè)計與制作，分離純化的技術(shù)方法及過程。培養(yǎng)結(jié)果表明：紅曲霉是一種耐酸、耐熱、耐乙醇的霉菌。純化后的紅曲霉在培養(yǎng)基上的菌落呈圓形，在30℃培養(yǎng)下，48h后開始生長，菌落初為白色，進而變?yōu)榈�粉色、紅色，直接紫紅、暗紅，但菌落邊緣為白色。培養(yǎng)12天后整個菌落呈深紅色。顯微鏡鏡檢觀察菌絲，菌絲有橫隔，多核，分支甚繁，分生孢子排列呈鏈狀，分生孢子頂端有球形閉囊殼。紅曲霉經(jīng)過分純后，針對發(fā)酵液培養(yǎng)基中不同碳源、氮源、pH分別進行單因素五水平的試驗，確定三個較好的水平，再進行三因素三水平的正交試驗以確定紅曲霉產(chǎn)紅曲色素的最佳條件。在單因素試驗中，碳源以玉米粉、淀粉、麥芽浸粉較好；氮源以硝酸鈉、大豆粉、蛋白胨較好；pH以3.2、4.0、 4.8較好。通過三水平三因素正交試驗，確定紅曲霉產(chǎn)紅曲色素的最佳培養(yǎng)條件為A1B2C2，即玉米粉、硝酸鈉、pH4.0為最佳組合。
 Ⅰ
關(guān)鍵詞：紅曲米；紅曲霉；分離；純化；平板稀釋法；紅曲色素；色價；液態(tài)發(fā)酵條件

Abstract：According to the nature of Monascus, the paper collected by sterile filtration of red yeast rice milled in Monascus spore suspension, using plate dilution method, realized from the simple separation of red yeast rice Monascus. According to the biological characteristics of Monascus, Summed up the principle of Monascus separation and separation media design and production, separation and purification method and process technology. Culture results showed that: Monascus is an acid, heat, alcohol-resistant fungus. After purification, the medium of Monascus the colonies were round, under cultivation at 30 ℃, 48h after the growth of the early colonies were white, and then into a light pink, red, direct purple, dark red, but the edge of the colony white. The colonies were cultured for 12 days and the colonies were dark red. Microscopic examination observed mycelium, mycelium with diaphragm, multi-core, branches very complex, spores arranged in chains, conidia are spherical cleistothesium top. After hours of Monascus pure, liquid medium for the fermentation of different carbon sources, nitrogen sources, pH were the single factors and five levels of testing, to determine the level of the three good, and then for three factors and three level orthogonal test to determine the production of Monascus red pigment in the best conditions. In the single-factor test, the carbon source for the corn flour, starch, malt extract powder are better; nitrogen as nitrate, soybean powder, peptone are better; pH to 3.2,4.0, 4.8 are better. Through three levels of the three-factor orthogonal experiment to determine the  production of Monascus red
 Ⅲ
 
pigment of the best medium for A1B2C2, is corn flour, sodium nitrate, pH4.0 was the best combination.
Key words: Red rice; Monascus; separation; purification; plate dilution method; red pigment; color value; liquid fermentation conditions.