嘌呤啤酒酵母選育

生物工程論文編號:SW158  論文字數:9667,頁數:24

目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1 嘌呤簡介 1
 1.2 啤酒中嘌呤的來源  1
 1.3 啤酒中嘌呤含量  2
 1.4 本課題研究的意義 2 
2．實驗部分 3
 2.1 實驗材料 3
 2.2 實驗方法 4
3. 結果與討論 8
 3.1 原生質體制備 8
 3.2啤酒的釀造 10
 3.3 嘌呤含量測定 10
 3.4 實驗小結 16
4 總結與展望 17
 4.1 總結 17
 4.2 展望 17
致謝 19
參考文獻 20

摘 要：本文通過高效液相色譜法測定了麥芽汁、發酵液及成品啤酒中的嘌呤含量，分析了嘌呤在發酵中產生的機理，提出有效的控制措施并加以改進。通過酵母菌原生質體紫外誘變，篩選產嘌呤低的菌株。以pH=3.85的0.02mol/L磷酸緩沖溶液作為液體流動相，波長254 nm 為檢測波長，進行檢測。實驗表明，原生質體形成條件：以1.5%的蝸牛酶作為破壁酶，0.7mol/L的蔗糖緩沖液為高滲壓穩定劑，在30℃恒溫水浴處理3-4h；紫外誘變條件：在25W紫外燈的照射下，進行誘變60s，使原生質體致死率達80%以上，再生率為5.6%，篩選菌株發酵，啤酒中的嘌呤含量較普通啤酒更低。該研究為開發低嘌呤啤酒提供了可靠的檢測方法和技術指導。
關鍵詞：酵母；原生質體；誘變；高效液相色譜；嘌呤

Abstract: In this paper, wort, fermenting liquor and beer were determined by HPLC, and the change of purines in fermentation process was analyzed, and proposed the effective control measure and to improve it. By the ultraviolet mutagenesis of the saccharomycetes protoplast,  screening produces the purine low strain. Take pH = 3.85 to the 0.02 mol/L phosphate buffer solution as the mobile phase liquid, and the Wavelength is 254 nm for test. The experiments show that, the conditions of protoplast’s formation is use the 1.5% of the snail-as-broken ,and 0.7mol / L of sucrose buffer for hypertonic pressure stabilizer, bath processing 3-4h in 30 ℃ water .And the conditions of UV-induced is in the ultraviolet light irradiation of 25W, Mutation 60s to make the protoplast death rate more than 80%,and the reproducibility is 5.6%. Repeated siftings and fermentation. The purine in beer content lower than the ordinary one. The research in this paper offered a reliable analytical method and the researching direction of low—purine beers．
 Keywords：Yeast ;Protoplast; Mutagenesis; Highly effective liquid chromatography; Purine