一株微生物新種生物學(xué)特性研究

生物工程論文編號:SW159  論文字?jǐn)?shù):13766,頁數(shù):30

目錄
摘要 I
Abstract II
目錄 III
1  緒論 1
1.1  微生物的發(fā)展 2
1.2  色素在食品中的應(yīng)用 3
1.3  生物礦化的特點 4
2  材料與方法 6
2.1  實驗材料 6
2.2 方法 7
3  實驗結(jié)果 12
3.1  菌種的分離純化 12
3.2  不同固體培養(yǎng)基中菌體生長情況的比較 13
3.3  不同液體培養(yǎng)基中菌體生長情況的比較 14
3.4  培養(yǎng)溫度對菌體生長的影響 14
3.5  pH對菌體生長的影響 15
3.6  He1產(chǎn)酶定性分析 16
3.7  抑菌作用 17
3.8  沙土管及其周圍沙土失重情況分析 17
3.9  沙土管及其周圍沙土中金屬元素的分析 18
3.10  He1生物礦化作用分析 21
4  總結(jié)與展望 24
致謝 25
參考文獻 26

摘要：寧夏沙漠地帶發(fā)現(xiàn)一種由微生物生長而凝結(jié)成的沙土管，對該微生物進行了分離純化，得到了該微生物的純培養(yǎng)，并命名為He1。對菌種的生長特性：對該菌的生長培養(yǎng)基、生長溫度、菌絲形狀、菌落形態(tài)等進行研究發(fā)現(xiàn)，該菌種的所需的生長培養(yǎng)基是查氏培養(yǎng)基，生長溫度為30℃，在顯微鏡下觀察到，菌種He1具有較長的菌絲，菌絲相互交纏，有橫隔，成竹節(jié)狀，基本無分支，有大量孢子產(chǎn)生，查資料確定該菌為放線菌。該菌能產(chǎn)大量的黑色素，在顯微鏡下，菌絲呈褐色。對提純的菌種進行生長特性、產(chǎn)酶特點、抑菌作用分析發(fā)現(xiàn)該菌蛋白質(zhì)水解能力很差卻具有良好的淀粉水解能力。沙土管金屬元素的分析中可以看出，沙土管中的Ca離子含量相當(dāng)多，沙土管中K 、Ca、Na、Mg金屬的含量分別是78.3 mg/kg、90.28 mg/kg、32.19 mg/kg、35.83 mg/kg，而在沙土中K 、Ca、Na、Mg金屬的含量分別是57.12mg/kg、20.66 mg/kg、18.59 mg/kg、11.78 mg/kg, Ca離子含量相差4倍多；生物礦化的模擬中在顯微鏡觀察到晶體顆粒，是CaCO3 晶體。

關(guān)鍵詞：沙土管；菌種分離純化；菌種的生長特性；生物礦化

Abstract：Our district that is in the desert in Ningxia discovers that a species may be a new microorganism, so carry on separating the early of the purification plate and picking a single colony to the germ species to grow with the purification germ.To the growth characteristic that germ grow:Carry on the research detection to the growth medium, the growth temperature, hypha shape, the colony appearance etc. of that germ, the growth medium need of that germ species is to check the surname medium, the growth temperature is 30℃, observing under the microscope, the germ species He1 has the longer hypha, the hypha hands over to tie up mutually, is horizontal separate, become the bamboo node form, basic have no branch, there is a great deal of spore creation, checking that germ of the data assurance as actinomycetes.That germ can produce a great deal of melanin, under the microscope, the hypha presents the brown.Have the good starch  water solution ability towards lifting the pure germ species to carry on growing the characteristic and producing the enzyme characteristics, the bacteriostasis analysis to discover that germ protein water  solution ability badly but.The analysis of the sandy soil tube metals chemical element in can see, the Ca ion content that is in the sandy soil tube is very many, the content of the K, Ca, Na, the Mg metals is the 78.3 mgs/ kg,90.28 mgs/ kg,32.19 mgs/ kg,35.83 mgs/ kg respectively in the sandy soil tube, but in the sandy soil the content of the K, Ca, Na, the Mg metals is the 57.12 mgs/ kg,20.66 mgs/ kg,18.59 mgs/ kg,11.78 mgs/ kg respectively, the Ca ion content differ 4 many;The organism mineral turns in the emulation to observe the crystal grain in the microscope, is a crystal of CaCO3.

Keywords: The sandy soil tube；The erm pecies solation purification;The growth characteristic of the erm species; The organism mineral turn