響應(yīng)面法優(yōu)化兩相體系發(fā)酵生產(chǎn)天然香料β-苯乙醇

生物工程論文編號(hào):SW157  論文字?jǐn)?shù):12590,頁(yè)數(shù):30

目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1  β-苯乙醇的性質(zhì)和用途 1
 1.2  β-苯乙醇的生產(chǎn)方法 1
 1.3  兩相體系 5
 1.4  市場(chǎng)前景 5
2. 實(shí)驗(yàn)部分 6
 2.1  實(shí)驗(yàn)材料 6
 2.2  實(shí)驗(yàn)方法 7
 2.3  實(shí)驗(yàn)設(shè)計(jì)方法 9
3. 結(jié)果與討論 16
 3.1  有機(jī)溶劑的選擇 17
 3.2  水/有機(jī)溶劑兩相體系與水相體系的反應(yīng)過(guò)程比較 17
 3.3  Plackett-Burman 設(shè)計(jì)結(jié)果與討論 18
 3.4  最陡爬坡實(shí)驗(yàn) 20
 3.5  響應(yīng)面方法優(yōu)化發(fā)酵條件 21
 3.6  驗(yàn)證試驗(yàn) 23
4．總結(jié)與展望 23
致謝 24
參考文獻(xiàn) 25

摘 要：考查了馬克斯克魯維酵母 AS2.1440 在有機(jī)溶劑/水兩相系統(tǒng)中生物轉(zhuǎn)化L-苯丙氨酸生產(chǎn) β-苯乙醇的過(guò)程。在單因素試驗(yàn)的基礎(chǔ)上，用Plackett-Burman 設(shè)計(jì)、最陡爬坡試驗(yàn)結(jié)合響應(yīng)面分析法對(duì)影響β-苯乙醇的因素進(jìn)行了研究，確定了最佳發(fā)酵條件為：兩相比例控制在油酸/水=2 : 3為宜；裝液量為64.46 mL/150mL三角瓶，培養(yǎng)基初始pH控制在5.0，溫度32.99℃，轉(zhuǎn)速160 r/min下轉(zhuǎn)化48 h，β-苯乙醇的產(chǎn)量可達(dá)1533±11 mg/L。與傳統(tǒng)水相相比，油酸-水兩相系統(tǒng)中Kluyveromyces marxianus AS2.1440 轉(zhuǎn)化 L-苯丙氨酸生產(chǎn) β-苯乙醇的產(chǎn)量提高了 102.91%。

關(guān)鍵詞：兩相體系；β-苯乙醇；響應(yīng)面方法； AS2.1440

Abstract: Bioconversion of L-phenylalanine to β-phenylethylalcohol with Kluyveromyces marxianus AS2.1440 was performed in an aqueous/organic solvent biphasic system. On the basis of single-factor tests, factors affecting Bioconversion of L-phenylalanine to β-phenylethylalcohol with Kluyveromyces marxianus AS2.1440 were eva luated by Plackett-Burman design, the path of steepest ascent and response surface methodology. The optimal bioconversion conditions are as follows: the concentration of L-phenylalanine was 4 g/L, the optimum values of volume ratio of organic phase to aqueous phase, reaction temperature, pH value and liquid volume were 2 : 3, 32.99℃, 5.0, and 64.46mL/250mL respectively. Under above optimum conditions, the content of β-phenylethylalcohol reached1533±11g/L48 h later, which increased by about 102.91% than that in monophasic aqueous system.

eywords：biphasic system; β-phenylethylalcohol; Response Surface Methodology; Kluyveromyces marxianus AS2.1440