積雪草苷的高效分離制備方法研究

生物工程論文編號:SW194  論文字數:10740,頁數:26

目  錄

中文摘要……………………………………………………………………………I
英文摘要……………………………………………………………………………II
目錄…………………………………………………………………………………III
1.緒論………………………………………………………………………………1
2.實驗部分…………………………………………………………………………6
 2.1實驗材料………………………………………………………………………6
 2.2實驗試劑與儀器設備…………………………………………………………6
 2.2.1試劑………………………………………………………………………6
 2.2.2儀器設備…………………………………………………………………6
 2.3色譜條件………………………………………………………………………6
 2.4實驗方法………………………………………………………………………7
 2.4.1  積雪草總苷溶解度考察………………………………………………7
 2.4.2  溶析劑選擇……………………………………………………………7
 2.4.3  考察溶析結晶分離規律………………………………………………8
 2.4.4 98%以上純度的積雪草苷制備…………………………………………9
3, 結果與討論………………………………………………………………………10
 3.1  溶劑篩選……………………………………………………………………10
 3.2  溶析劑篩選…………………………………………………………………10
 3.3  溶析劑種類對分離效果的影響……………………………………………12
 3.4  溶劑對分離效果的影響……………………………………………………15
 3.5  積雪草總苷濃度對分離效果的影響………………………………………17
 3.6  高純度積雪草苷制備………………………………………………………19
4. 總結與展望………………………………………………………………………19
 致謝…………………………………………………………………………………21
 參考文獻……………………………………………………………………………22

摘要  采用溶析結晶法從積雪草總苷中高效制備高純度積雪草苷。通過考察不同積雪草總苷于各種溶析劑下的溶解度，以及考察了不同溶劑—溶析劑體系下從積雪草總苷分離制備積雪草苷的規律。得到了98.1%以上純度的積雪草苷。該方法工藝簡單，大大地降低了生產成本以及生產過程中的能源消耗，減少了污染排放。

關鍵詞  積雪草；積雪草苷；溶析結晶

Abstract   A crystallization method is applied to separate asiaticoside from  total glucosides of Centella asiatica. Through the study of different solubility of total glucosides of Centella asiatica under a variety of melting-out agent and the resolution in different solvents - melting-out agent system were observed. More than 98.1% purity asiaticoside was obtained. The method is simple process, lower the prime cost and energy consumption, reducing pollution emissions.

Keywords   Centella asiatica; Asiaticoside; Crystallization