疣粒野生稻來源的水稻抗白葉枯病單染色體片段滲透系的篩選

生物工程論文編號(hào):SW195  論文字?jǐn)?shù):10576,頁(yè)數(shù):24

目  錄
摘要 I
Abstract. II
目  錄 III
1.緒論 1
1.1 水稻白葉枯病的致病及其抗病機(jī)理 1
1.2 水稻白葉枯病的病理基礎(chǔ)知識(shí)及其對(duì)水稻的危害 2
1.2.1水稻白葉枯病的發(fā)病癥狀 2
1.3 水稻抗白葉枯病研究進(jìn)展 3
1.4 分子標(biāo)記的研究 4
1.4.1 SSR技術(shù)的特點(diǎn) 5
1.4.2 STS標(biāo)記（sequence tagged site，SSR序標(biāo)位) 6
1.4.3 SSR標(biāo)記（simple sequence repeats,SSR 簡(jiǎn)單重復(fù)序列） 6
1.4.4 SSR 標(biāo)記技術(shù)的基本原理 6
1.4.5 SSR及STS 標(biāo)記技術(shù)程序 7
2.實(shí)驗(yàn)內(nèi)容 7
2.1實(shí)驗(yàn)材料和方法 7
2.1.1 實(shí)驗(yàn)材料 8
2.1.2 水稻DNA的提取 9
2.1.3 制膠、電泳及檢測(cè) 9
2.1.4 DNA片段的PCR擴(kuò)增 10

3.結(jié)果與討論 11
3.1 親本電泳對(duì)照?qǐng)D 12
3.2 討論 14
4.總結(jié)與展望 14
致謝 16
參考文獻(xiàn) 17
附錄 19

摘要：疣粒野生稻對(duì)水稻白葉枯病高抗，通過體細(xì)胞雜交將其抗性導(dǎo)入高產(chǎn)栽培稻大粒香中。經(jīng)QTL定位，將高抗的雜交后代的抗性位點(diǎn)定位到1,3,5號(hào)三條染色體上，為了對(duì)這些抗性位點(diǎn)精細(xì)定位并克隆抗病基因，經(jīng)多代回交構(gòu)建了抗病雜交后代與感病親本的高代回交群體，利用這些群體結(jié)合QTL連鎖的分子標(biāo)記進(jìn)行包含抗性位點(diǎn)的單染色體片段滲透系的篩選，為抗性QTL位點(diǎn)的精細(xì)定位及基因克隆奠定基礎(chǔ)。本實(shí)驗(yàn)就是對(duì)雜交群體中1號(hào)染色體上包含抗性QTL位點(diǎn)在內(nèi)的單染色體片段滲透系的篩選。先通過電泳消除495個(gè)個(gè)體中3號(hào)及5號(hào)染色體上的抗性影響，使其擁有純合的感病基因，繼而對(duì)1號(hào)上的抗性位點(diǎn)進(jìn)行研究，篩選包含抗性QTL位點(diǎn)在內(nèi)的單染色體片段滲透系。
關(guān)鍵詞：水稻；單染色體片段；白葉枯病

Abstract： wild rice with high resistance to rice bacterial blight, by somatic cell hybridization to brought its high resistance into cultivated rice in a large grain incense. By QTL location, the high resistance of the hybrid offspring of the resistance loci in No. 1,3,5. in order to fine mapping of these resistance loci and cloning of resistance genes,was constructed by resistance disease and susceptible hybrids groups, these groups combined use of molecular markers linked to QTL for resistance loci contain a single chromosome segment penetration system of selection for the resistance locus QTL Fine Mapping and Cloning basis. The experiment is on chromosome 1 hybrid population's resistance gene screening. First by electrophoresis elimination of 500 individuals NO.3 and NO.5 Effects of resistance on chromosome so that it has a homozygous susceptible genes. Then on the NO.1's Resistance to research.
Key words: Rice; single chromosome segment; blight