水翁花主要成分的抗氧化活性研究

制藥工程論文編號(hào):ZY011    論文字?jǐn)?shù):9856,頁(yè)數(shù):20

目  錄
中文摘要 I
英文摘要 II
目錄 III
1 緒論 1
 1.1 前言 1
 1.2 水翁花 2
 1.3 抗氧化劑概念及其作用機(jī)理 2
 1.4 常用評(píng)價(jià)抗氧化活性的方法 3
2 實(shí)驗(yàn)部分 8
 2.1 實(shí)驗(yàn)材料與方法 8
3 結(jié)果與討論 11
 3.1 超氧陰離子自由基清除法   11
     3.2 DPPH自由基清除法 12
     3.3 羥自由基Fenton反應(yīng)比色法 13
     3.4 結(jié)論 13
總結(jié)與展望 15 
致謝 16
參考文獻(xiàn) 17

摘要:在正常生理情況下，活性氧的產(chǎn)生與清除可維持在一個(gè)有利無(wú)害的極低水平。活性氧的重要性在過(guò)去十年已被日益關(guān)注，根據(jù)現(xiàn)有的研究報(bào)道，活性氧在組織和器官表現(xiàn)出雙重性作用，不僅能極力與脂質(zhì)過(guò)氧化,從而導(dǎo)致食品腐爛,而且還能引發(fā)一系列疾病，如細(xì)胞衰變、致突變、致癌、冠心病、糖尿病、神經(jīng)退化等。植物中具有許多生物活性如抗衰老、抗氧化、抗癌的化學(xué)成分。水翁花中提取得到的兩個(gè)主要成分，2′,4′-二羥基-6′-甲氧基-3′,5′-二甲基查耳酮以及烏索酸,這2個(gè)化合物具有良好的抗氧化活性，因此通過(guò)超氧陰離子清除法、DPPH自由基清除法、羥自由基Fenton反應(yīng)比色法與標(biāo)準(zhǔn)品還原能力比較實(shí)驗(yàn)等實(shí)驗(yàn)考察這二個(gè)化合物的抗氧化活性。
關(guān)鍵字：抗氧化劑；黃酮類化合物；DPPH；活性氧；水翁花
 
Abstract:Under normal physiological conditions,the production of reactive oxygen species(ROS) can be beneficial and harmless in the relatively low level. The importance of ROS has attracted increasing attention over the past decade. Modern theories of ROS have revealed that ROS play a dual role in organisms. ROS are not only strongly associated with lipid peroxidation, which lead to the deterioration of the food, but also involved in the development of a variety of diseases including cellular aging、mutagenesis、carcinogenesis、coronary heart disease、diabetes and neurodegeneration. Plants have many phytochemicals with various bioactivities,including antioxidant,anti-inflammatory and anticancer activities. The two major components,2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone and ursolic acid isolated from Cleistocaly operculatus,were investigated for their antioxidant activities. Superoxide anion radical scavenging assays,1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and Hydroxyl radicals in Fenton Reaction by colorimetric assay were carried out to eva luate the antioxidant potential of the two compounds.
Keywords: Antioxidants; Flavonoids; DPPH; Reactive oxygen species; Cleistocaly operculatus