Abstract: Enzyme, as a biological catalyst, is widely used in food processing, pharmaceuticals and fine chemical industries for its high selectivity, mild reaction, non-pollution and other good characteristics. As people pay more attention to the environmental protection, the application of enzyme will be more concerned. However, there are many shortcomings of free enzyme, such as poor stability, easy deactivation, non-reuse, difficultly purification and so on. It is more difficult to be widely used in the field of industry. Under these conditions, the concepts and techniques of immobilization of enzyme are mentioned and developed. In addition, how to make use of the natural polymer carrier or to make use of some advanced technology, such as supercritical technology, nanometer technology, membrane technology ,to immobilize enzyme have become interesting topics on enzymatic engineering.
This article is mainly study the technology of immobilization of amylase on magnetic nanoparticles. Amylase was immobilization on magnetic nanoparticles. And then try to exam the immobilization yield of the immobilized enzyme,and the enzyme activities of immobilized enzyme and free enzyme to demonstrate some characters of immobilized enzyme.
Keywords：immobilization ; magnetic nanoparticles; amylase; activity
