脂肪酶催化的knoevenagel反應研究

制藥工程論文編號:ZY039    論文字數(shù):9169,頁數(shù):28

目  錄
中文摘要 I
英文摘要 II
目    錄 III
1. 緒論 1
1.1 酶的混雜性催化 1
1.2 研究的背景 2
1.3 研究的意義 2
1.4 knoevenagel反應的簡介 3
1.5脂肪酶的簡介 4
1.6總  結 7
2. 實驗部分 8
2.1實驗原理 8
2.2實驗材料 8
2.3實驗步驟 8

3. 結果與討論 10
3.1 結果 10
3.2 討論 19
4. 總結與展望 19
致    謝 22
參考文獻 23

摘 要：酶的催化混雜性,是當今催化領域研究的熱點，它是指同一個催化核心具有催化兩個或者兩個以上不同類型的化學反應的能力�？疾烀傅幕靵y性催化，為進一步研究酶的結構與功能的關系和開發(fā)酶的新功能提供了新的思路, 同時為蛋白質科學研究開辟了新的空間。本次實驗通過篩選數(shù)種酶對 knoevenagel 反應的催化能力，第一次發(fā)現(xiàn)豬胰脂肪酶對這個反應具有很好的催化作用。并且，我們發(fā)現(xiàn)脂肪酶在催化這個反應的同時，還可以完成它原有的酯交換的能力。通過對這兩種反應的動力學研究，我們發(fā)現(xiàn)了脂肪酶催化 knoevenagel 反應的能力遠遠強于其固有的催化酯交換的能力。本次實驗不僅擴大了酶的催化領域，而且對酶的進化具有一定的意義。
關鍵詞：脂肪酶；混亂性催化；動力學；酯交換。

Abstract:Nowadays，the hybridity of catalysis of enzyme is the focus areas of catalytic current. It is a capability which can catalyst two or more different types of chemical reactions by the same catalytic core. The expedition of the hybridity of catalysis of enzyme，provides a new way of thinking for father studying of the relationship between structure and function of the enzyme and developing the new functional of enzymes. By screening several enzymes which have the ability of catalytic knoevenagel reaction，we find the PLL has a very good catalysis to this reaction at the first time. In the reaction，we find that Lipase can not only catalytic the reaction，but also execute the original reaction of transesterification. Through the study of Dynamics of these two reactions，we find that the capability of the enzyme catalytic the reaction is faster than the reaction of transesterification. The experiment not only expanded the field of enzyme catalysis，but also have a certain significance to the evolution of enzyme.

Keywords:Lipase;Chaotic nature of catalysis;Dynamics;Transesterification.