蘆氟沙星與DNA相互作用的研究

制藥工程論文編號:ZY051    論文字數:11332,頁數:25

目   錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1  概述 1
 1.2 DNA與金屬物質的相互作用 2
 1.3 DNA與非金屬小分子的相互作用 4
 1.4 藥物分子與DNA相互作用的作用本質及影響因素 5
 1.5 蘆氟沙星的性質 6
 1.6 研究方法 7
2. 實驗部分 8
 2.1 儀器與試劑 8
 2.2 試驗方法 8
3. 結果與討論 10
 3.1 蘆氟沙星和DNA相互作用的熒光光譜及熱力學研究  10
 3.2 蘆氟沙星和DNA相互作用的鍵和模式 11
4．總結與展望 16
   4.1 實驗總結 16
   4.2 展望 16
致謝 18
參考文獻 19

 摘要：本論文通過熒光光譜、紫外光譜、粘度測定等研究蘆氟沙星與DNA的相互作用。分別測定了293K和310K兩個不同溫度下二者作用的猝滅常數KSV，探討了其熒光猝滅的機理。在粘度測定中，蘆氟沙星與DNA的作用沒有引起DNA雙螺旋骨架的扭曲變化，而且在紫外吸收光譜中，DNA 的加入并沒有對蘆氟沙星的吸收峰產生影響，未出現等吸收點；隨著蘆氟沙星濃度的增加，EB-DNA熒光強度有所減弱，鍵合飽和時，體系的熒光強度未達50%，故蘆氟沙星未發生嵌插作用競爭EB的結合位點，并且在KI的熒光猝滅中，KI對單純蘆氟沙星及與CT-DNA共存的蘆氟沙星兩種體系的猝滅程度幾乎一致，蘆氟沙星并未受到DNA堿基對的保護。實驗結果表明，DNA對蘆氟沙星的熒光猝滅常數為2.89×103L ·mol-1，ΔH=-12.91KJ·mol-1，蘆氟沙星與CT-DNA之間不是靜電作用，也不是嵌插鍵合，而是溝槽鍵合。
 關鍵詞：蘆氟沙星；DNA；熒光猝滅；溝槽鍵合 
 
 Abstract: Fluorescence spectroscopy, UV spectroscopy, viscosity measurement          and other experiments was used to study interaction of rufloxacin with the DNA .Under the temperature of 293K and 310K , the quenching constants  were measured to discusse the mechanism of fluorescence quenching. In the determination of the viscosity experiment, rufloxacin did not distort the DNA backbone. In the UV absorption spectrum,equal absorption point did not appear by the addition of rufloxacin With the increasing rufloxacin concertration,EB-DNA fluorescence intensity was weakened less than 50% suggested that rufloxacin did not compete the EB binding sites.In  the fluorescence quenching of KI experiment, the results were almost same under the simply rufloxacin system and the coexistence of CT-DNA with rufloxacin system.The result suggested that rufloxacin was protected by the DNA bases . In conclusion, rufloxacin interacted with CT-DNA with the quenching constant of 2.89×103L ·mol-1. The interaction is exothermic with a Van’t Hoff enthalply of -12.91KJ·mol-1. Rufloxacin interacted  with CT-DNA in the mode of groove binding .
Key words: Rufloxacin; DNA; Fluorescence quenching; Groove bonding