響應面法優化提取蒲公英黃酮工藝研究

制藥工程論文編號:ZY053    論文字數:9736,頁數:21

目  錄
中文摘要 I
英文摘要 II
目錄 III
1．緒論 1
   1.1蒲公英介紹以及黃酮類化合物生物活性介紹 1
   1.2響應面分析法的介紹 3
2．實驗部分 5
 2.1材料與儀器 5
 2.2實驗方法 5
3．結果與討論 7
 3.1提取時間對蒲公英黃酮提取率的影響 7
 3.2提取溫度對蒲公英黃酮提取率的影響 7
 3.3乙醇濃度對蒲公英黃酮提取率的影響 8
 3.4料液比對蒲公英黃酮提取率的影響 9
 3.5黃酮得率回歸模擬建立及顯著性檢驗 9
 3.6蒲公英黃酮提取的響應面分析與優化 12
 3.7蒲公英黃酮最佳提取條件的確定和試驗驗證 14
4．總結與展望 16
致謝 17
參考文獻 18

摘要：本研究采用乙醇提取法從蒲公英中提取黃酮，考查了乙醇體積分數、料液比、提取溫度和提取時間等因素對蒲公英黃酮提取效果的影響。為確定蒲公英中黃酮類成分乙醇提取的最佳工藝條件，以黃酮得率為指標，在單因素試驗基礎上采用響應面法對主要工藝參數進行優化并得到回歸模型。方差分析結果表明：回歸模型較好地反映了蒲公英黃酮得率與提取時間、提取溫度、乙醇體積分數和料液比的關系；最佳工藝條件為提取時間52min、提取溫度65℃、乙醇體積分數58%、料液比1∶20g/ml。此工藝條件下提取蒲公英黃酮得率為8.16%（以蘆丁計），回歸模型的預測值與實際值的相對誤差為1.3%。該優化工藝提高了黃酮的提取率，取得了較好的成效。
關鍵詞：蒲公英；黃酮；提取工藝；響應面法

Abstract: The ethanol extraction of flavonoid from Taraxacum mongolicum were carried out. The effects of concentration of ethanol, ratio of solvent to raw material， temperature and extracting time on extraction effect of flavonoid from Taraxacum mongolicum were discussed. With the aim of optimization of the ethanol process for extracting flavonoid from Taraxacum mongolicum, a central composite design(CCD) experiment was conducted and the response surface methodology(RSM) based on single factor was employed to investigate the influence of four main factors on the yield of flavonoid, and to establish a regression model. Regression coefficient and variance analysis show the regression model fits the actual process well. The obtained optimum extraction parameters are extracting time 52min, temperature 65℃, concentration of ethanol 58%, and ratio of solvent to raw material 1:20. The flavonoid yield with the optimized procedure is 8.16%(as rutin). The experimental values agree with those predicted from the regression model within a relative error of 1.3%, Applying this extracting technology, the flavonoid content is increased, the results showed that this process is reasonable and practicable.
Key words: Taraxacummongolicum; flavonoids; extraction technology; response surface methodology