夫西地酸的分離純化研究

制藥工程論文編號:ZY068    論文字數:7421,頁數:20

 目  錄
中文摘要 I
英文摘要 II
目錄 III
1．緒論 1
1.1夫西地酸概述 2
1.2大孔吸附樹脂概述 2
1.3總結 3
2．實驗部分 3
2.1夫西地酸標準品在不同溶劑下的溶解度實驗 3
2.2夫西地酸發酵液的分離研究 4
2.3夫西地酸的HZ-816樹脂柱層析提純 5
3．結果與討論 7
3.1夫西地酸標準品在不同溶劑下的溶解度實驗 7
3.2夫西地酸發酵液的分離研究結果 7
3.3夫西地酸的HZ-816樹脂柱層析提純結果 9
3.4實驗結果總結 12
4．總結與展望 13
4.1總結 13
4.2展望 13
致謝 15
參考文獻 16

摘要 :本論文研究建立夫西地酸發酵液的分離純化工藝，夫西地酸是一種窄譜、高效的抗生素，對預防和治療葡萄球菌感染療效顯著。根據夫西地酸的溶解特性，采用過濾、干燥、萃取和柱層析等技術相結合，設計不同的工藝路線，通過對酸堿調節試劑、流動相梯度、柱層析樹脂的選擇，采用高效液相色譜法檢測產物的純度和收率作為評價指標，得到的最優工藝流程。該工藝其產品的收率為44.53%，組分含量為98.23%。該方法簡便易行，準確可靠，能有效控制產品的質量，可用于夫西地酸的工業生產。
關鍵詞：夫西地酸；分離；柱層析；高效液相色譜法

Abstract:This study was to establish the technology process of the separation and purification of the fucidic acid. Fusidic acid is an efficient narrow-spectrum antibiotic. The treatment of staphylococcus aureus infection is remarkable. According to the dissolution characteristics of fusidic acid, we used the filtering, drying, extraction, column chromatography and so on to design different technics processes. HPLC was used to check the purity and yield of the products to optimize the production process of the fucidic acid. Results suggested that the yield was 44.53% and the component concentration was 98.23%. This technology process was simple, accurate, reliable and effective for the manufacturing production.
Keywords: Fucidic acid; Separation; Column chromatography; HPLC