超臨界萃取及洋蔥油抑菌活性研究

制藥工程論文編號:ZY070    論文字數(shù):9090,頁數(shù):31

目  錄
中文摘要...........................................................Ⅰ
英文摘要...........................................................Ⅱ
目錄...............................................................Ⅲ
1.緒論..............................................................1
  1.1 特點..........................................................1
  1.2 組成成分......................................................1
  1.3 保健功效......................................................1
  1.4 超臨界原理....................................................3
  1.5 抑菌活性......................................................3
2.實驗部分..........................................................4
  2.1 實驗材料......................................................4
  2.2 試驗方法......................................................5
 3.結果與討論........................................................7

3.1 超臨界萃取....................................................7
  3.2 抑菌譜........................................................8
  3.3 最低抑菌濃度與最低殺菌濃度....................................9
4.總結與展望.......................................................13
致謝...............................................................14
參考文獻...........................................................15
附錄...............................................................16

摘要:通過利用超臨界二氧化碳流體萃取技術萃取洋蔥油，進而采用牛津杯法對其進行抑菌譜研究，培養(yǎng)基稀釋法分別對各菌種進行最低抑菌濃度、最低殺菌濃度研究。從而獲得洋蔥油對金黃色葡萄球菌、大腸桿菌、枯草芽孢桿菌、酵母菌類都有明顯抑菌效果，而對寧佐美曲霉、黑曲霉、紅曲霉、三孢布拉氏霉菌等真菌類沒有抑菌效果。分別對應金黃色葡萄球菌、大腸桿菌、枯草芽孢桿菌、酵母1號的最低抑菌濃度為0.00029、0.00036、0.00065、0.000072g/ml，最低殺菌濃度為0.0014、0.00072、0.00072、0.000072g/ml。

關鍵詞：超臨界萃取；洋蔥油；抑菌譜；最低抑菌濃度；最低殺菌濃度

Abstract：With using supercritical fluid carbon dioxide extraction technology ，onion oil were extracted from onion, and do the Antibacterial spectrum of research by Oxford cup method, and the medium dilution method were carried out on the bacteria minimal inhibitory concentration, minimum bactericidal concentration studies. The results of onion oil on the Staphyloccocus aureus , E. coli, Bacillus subtilis, yeast have a clear inhibitory effect ,but there is no inhibition on the effect of Aspergillus usamii，Aspergillus niger,Monascus purpureus，Blakeslea trispora，respectively.Corresp-
onding Staphyloccocus aureus , E. coli, Bacillus subtilis, yeast 1, the minimum inhibitory concentration for 0.00029,0.00036,0.00065，0.000072g/ ml, the minimum bactericidal concentration for 0.0014,0.00072,0.00072,0.000072 g/ ml.
Key Words: Supercritical fluid extraction；Onion oil； Antibacterial spectrum； Minimum inhibitory concentration； Minimum bactericidal concentration