烏藥指紋圖譜的初步研究

制藥工程論文編號:ZY091    論文字數(shù):4162,頁數(shù):23  附任務書，開題報告，文獻綜述，外文翻譯 PPT

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目錄
中文摘要……………………………………………………………………………Ⅰ
英文摘要……………………………………………………………………………Ⅱ
目錄…………………………………………………………………………………Ⅲ
1.緒論………………………………………………………………………………1
2.實驗部分…………………………………………………………………………2
 2.1 樣品、儀器和試劑…………………………………………………………2
 2.1.1 樣品…………………………………………………………………2
 2.1.2 主要儀器和設備……………………………………………………2
 2.1.3試劑…………………………………………………………………2
 2.2 實驗方法……………………………………………………………………2
 2.2.1 色譜條件……………………………………………………………2
 2.2.2 樣品溶液的制備……………………………………………………2
 2.2.3 樣品分析……………………………………………………………2
 2.2.4 重復性實驗…………………………………………………………2
3 結果與討論………………………………………………………………………3
 3.1 色譜條件法的確定…………………………………………………………3
 3.1.1 檢測波長的考察……………………………………………………3
 3.1.2 流動相體系的考察…………………………………………………8
 3.1.3 小結…………………………………………………………………10
 3.2 樣品溶液的制備……………………………………………………………10
 3.2.1 提取溶劑的考察……………………………………………………10
 3.2.2 提取方式的考察……………………………………………………13
 3.3 重復性考察…………………………………………………………………14
 3.4 樣品分析……………………………………………………………………16
4結論與建議………………………………………………………………………20
致謝…………………………………………………………………………………22
參考文獻……………………………………………………………………………23

摘要: 本論文主要研究用高效液相色譜法（HPLC）建立烏藥莖、直根及塊根的指紋圖譜。分別考察了檢測波長、流動相、樣品提取溶劑及提取方式對烏藥指紋圖譜的影響,實驗結果表明：檢測波長在235nm時出峰最多；流動相為乙腈－水（56:44，v/v）時，能比較好的體現(xiàn)指紋圖譜整體性的特點；提取溶劑為無水乙醇效果較好；提取方式為加熱回流時提取效果較優(yōu)。根據上述實驗結果，初步建立了烏藥莖、直根及塊根的指紋圖譜。
關鍵詞: 烏藥；指紋圖譜；HPLC

Abstract：In the dissertation, we established the fingerprints of Lindera aggregata stem and root by HPLC. In our research, we found that detection wavelength, mobile phase, solvent and extraction method were main facters in the fingerprints of Lindera aggregate. The conditiongs of HPLC were optimized. Lindera aggregata was refluxed in ethanol. The spectra appears more comprehensive in wavelength of 235 nm. It could reflect the fingerprints better while acetonitrile-water(56:44 v/v) was used. According to the conditions, we initially established the fingerprints of Lindera aggregata stem and root.
KeyWords：Lindera aggregata； fingerprint；HPLC