茶皂素提取工藝的研究

制藥工程論文編號:ZY093    論文字數:7037,頁數:17

目錄
中文摘要 ..........................................................I
英文摘要.......................................................... II
目錄  ........................................................... III
1. 緒論  ...........................................................1
   1.1 引言  .......................................................1 
 1.2 茶皂素的主要提取方法 ........................................2
 1.2.1  正丁醇萃取法 ........................................2
 1.2.2  重結晶法 ............................................2
 1.2.3  吸附法 ..............................................2
 1.2.4  聚酰胺柱層析法 ......................................3
 1.2.5  硅膠柱層析 ..........................................3
 1.2.6  離子交換樹脂法 ......................................3
 1.2.7  絮凝劑法.............................................3
 1.2.8  超濾膜法.............................................3
 1.2.9  大孔樹脂法...........................................4
 1.3 皂甙的主要結構鑒定方法.......................................4
2. 實驗部分.........................................................5
 2.1 試劑與儀器...................................................5
 2.1.1  材料..................................................5
 2.1.2  主要試劑...............................................5
 2.1.3  主要儀器設備...........................................5
 2.2 實驗步驟....................................................... 6
    2.2.1  大孔吸附樹脂的預處理..................................6
   2.2.2  大孔吸附樹脂的再生................................... 6
   2.2.3  大孔吸附樹脂吸附..................................... 6
       2.2.4  高效液相色譜分析..................................... 6
3. 結果與討論.......................................................7
   3.1 影響茶皂素收率的因素分析.....................................7
    3.1.1  乙醇濃度對茶皂素的收率的影響..........................7
    3.1.2  吸附次數的影響........................................7
    3.1.3  過柱吸附速度的影響....................................8
    3.1.4  吸附過程最佳工藝參數的確定............................9
 3.2 茶皂素含量分析.............................................. 9
 3.3 討論........................................................ 9
4. 總結與展望..................................................... 10
致謝...............................................................12
參考文獻...........................................................13

摘 要：油茶是山茶科植物屬植物油,我國是世界上油茶籽產量最大、品種最多的國家。油茶籽餅中含有豐富的三萜皂甙。油茶皂甙是一種良好的表面活性劑，有較好的洗滌效果，耐高濃度電解質能力也較好，乳化、起泡、抗靜電能力都比較強，可用于碳酸飲料的發泡劑、高級洗滌劑、滅火劑、乳化劑；油茶皂甙還有良好的藥用價值，具有溶血、消炎、祛痰、抗真菌、抗突變的作用。本論文對從茶粉末中提取茶皂素的生產工藝進行研究，對影響茶皂素收率的因素進行了分析討論，優化純化提取工藝。并用高效液相色譜儀對茶皂素的含量進行定量分析。得含量為94%。
關鍵詞：油茶籽餅；茶皂素 ；提取；高效液相色譜

Abstract：Camellia is a vegetable oil, which is the world's largest varieties camellia oil in China. Triterpenoid Saponins is rich in camellia oil cake. Camellia Saponin is a good surfactant, a better washing effect of high concentrations of electrolyte resistance ability to better emulsification, foaming, anti-static capacity than the stronger of carbonated drinks can be used to foam, senior scullery agents, fire extinguishing agent, emulsifier，the medicinal value of Saponin are good with hemolytic, anti-inflammatory, expectorant, anti-fungal, anti-mutation effect. In this paper, study the production process of Tea Saponin extracting from the tea saponin, and discuss the impact of yield factors and optimize the extraction and purification. The quantitative analysis of Tea Saporin by reverse phase HPLC is taken．The results shown content is 94%.
Keywords：Camellia oil；Tea Saponin；Extract；HPLC