禽源不同血清型大腸桿菌對(duì)同一藥物敏感性檢測(cè)

食品科學(xué)論文編號(hào):SP028 論文字?jǐn)?shù):11749,頁(yè)數(shù):23

目錄
摘要 1
Abstract 1
1  前 言 2
2  材料與方法 4
2.1  材料 4
2.1.1  病料 4
2.1.2  培養(yǎng)基 4
2.1.3  普通細(xì)菌生化鑒定管 4
2.1.4  試劑 4
2.1.5  儀器與設(shè)備 4
2.1.6  實(shí)驗(yàn)用具 4
2.1.7  實(shí)驗(yàn)藥物 4
2.2  方法 5
2.2.1  實(shí)驗(yàn)所需各種培養(yǎng)基的配制 5
2.3  操作步驟 6
2.3.1  技術(shù)路線和檢驗(yàn)程序 6
2.3.2  病料采集、疑似大腸桿菌菌樣純培養(yǎng)、初步鏡檢 7
2.3.3  確定實(shí)驗(yàn)用大腸桿菌 8
2.3.4  純化培養(yǎng)和增殖培養(yǎng) 9
2.3.5  純化菌種鏡檢和大腸桿菌顯色培養(yǎng) 9
2.3.6  生化鑒定 10
2.3.7  血清型鑒定 10
2.3.8  藥物敏感性試 11
3  結(jié)果與分析 11
3.1  病料采集 11
3.2  初步鏡檢結(jié)果 12
3.3  菌種篩選結(jié)果與菌種純化增殖培養(yǎng)結(jié)果 12
3.4  純化菌種鏡檢結(jié)果和顯色培養(yǎng)結(jié)果 14
3.5  生化鑒定結(jié)果 15
3.6 血清型鑒定結(jié)果 17
3.7  藥敏試驗(yàn)結(jié)果 19
4  討 論 22
參  考  文  獻(xiàn) 23

摘要：本實(shí)驗(yàn)通過(guò)對(duì)禽源不同血清型大腸桿菌對(duì)同一藥物的敏感性檢測(cè)的研究，探討大腸桿菌不同血清型對(duì)多種藥物敏感性，用來(lái)科學(xué)指導(dǎo)臨床用藥，治療禽大腸桿菌病。實(shí)驗(yàn)共有25株菌種鑒定出9個(gè)菌株6血清型血清型，進(jìn)行26種藥物藥敏試驗(yàn)，結(jié)果得出6個(gè)血清型對(duì)先鋒Ⅴ、氯霉素、菌必治等10種藥物均表現(xiàn)高敏，對(duì)另外10種藥物均表現(xiàn)耐藥性，還有6種藥物敏感性表現(xiàn)不一致，根據(jù)試驗(yàn)結(jié)果治療大腸桿菌病推薦使用高敏的10種藥物及敏感性較高的氧氟沙星、卡那霉素。
關(guān)鍵詞  大腸桿菌  血清型  藥敏試驗(yàn)   

     Sensitivity Testing of Different Serotypes of Avian E. coli Bacteria for the Same Drug
    

Abstract：    This paper studied the sensitivities of different serotypes of avian
E. coli for the same drug by the drug sensitivity tests, and provide theoretical basis for clinical treatment.  In this experiment, 6 serotypes of the bacteria were gained from nine strains of 25 species, and the high-sensitivity drugs were screened from 26 antibiotics for the six E. coli bacteria.  The results showed that 6 serotypes of the bacteria were high-sensitivity for 10 kinds of drugs such as pioneer V, chloramphenicol and junbizhi, showed resistance to 10 kinds of drugs, and were inconsistent sensitivity for the other 6 kinds of drugs.  From those results, 10 high-sensitivity drugs, ofloxacin and kanamycin were recommended to use in treating the E. coli disease.
Key words：E. coli; Serotype; Drug Sensitivity Test