杏鮑菇60Co-γ射線輻照誘變劑量研究

食品科學論文編號:SP045  論文字數:11500,頁數:25,附任務書,開題報告,文獻綜述,外文翻譯

 
 目   次
摘要…………………………………………………………………………………Ⅰ
目次…………………………………………………………………………………Ⅲ
1前言…………………………………………………………………………………1
1.1杏鮑菇概述及研究概況………………………………………………………2
1.2 珍稀食用菌的市場和發展趨勢………………………………………………3
 1.3 食用菌育種方法簡介…………………………………………………………4
2材料與方法…………………………………………………………………………7
 2.1實驗材料………………………………………………………………………7
  2.1.1誘變材料……………………………………………………………………7
 2.1.2 培養基………………………………………………………………………7
 2.2實驗方法…………………………………………………………………7
 2.2.1  菌絲培養 …………………………………………………………………7
  2.2.2  60Co-γ射線輻照處理………………………………………………………8 
 2.2.3  恢復培養…………………………………………………………………8
2.2.4  萌發后宏觀微觀形態觀察………………………………………………8 
2.2.5  菌絲培養特性試驗………………………………………………………8 
2.2.6  變異菌株篩選……………………………………………………………9
2.2.7  突變菌株液體發酵菌絲體累積量試驗…………………………………9
3 結果與分析………………………………………………………………………10
3.1  萌發后宏觀形態特征……………………………………………………10
3.2  萌發后微觀形態特征……………………………………………………14 

3.3  菌絲培養特性試驗結果…………………………………………………16

 3.4  拮抗實驗結果……………………………………………………………17
 3.5  發酵菌絲體累積量試驗結果……………………………………………18
4討論………………………………………………………………………………18
參考文獻……………………………………………………………………………19

摘要：杏鮑菇(Pleurotus eryngii)是著名食用菌之一，因其蛋白質多糖含量豐富，并含有多種生理活性物質，深受國內外消費者的青睞，并歸為我國大力推廣的珍稀食用菌品種之一。其生產周期短、見效快，經濟效益相對較高，近年來種植面積不斷擴大。
 為了獲得杏鮑菇優質高產菌株，本實驗采用60Co-γ射線對杏鮑菇的孢子、菌絲、子實體三種材料進行輻照處理，觀察對它們的誘變效應。通過對恢復萌發后菌絲的顯微結構對比、菌落生長形態觀察對比、生長速度測定、拮抗性實驗等，篩選出突變菌株。
 實驗結果表明，杏鮑菇菌絲的最佳劑量為500Gy左右；輻照劑量為200Gy時，孢子的致死率達70%；子實體的半致死劑量為1000Gy。通過拮抗試驗和液體發酵菌絲體累積量試驗，發現輻照后菌株與原始菌株比較，菌絲積累量差異并不明顯，但實驗結果初步確定了半致死劑量，為進一步研究杏鮑菇的誘變育種提供了理論上的依據。
 
關鍵詞：杏鮑菇；60Co-γ射線；誘變；突變株

Study of Mutagenic Dosage of 60Co-γ Ray Irradiated on Pleurotus eryngii

Abstract：Pleurotus eryngii is under the fungi door, Pleurotus. its meat hypertrophy like abalone and with almond flavor, which was named after Pleurotus eryngii. Its proteins and polysaccharides content is very rich, and contains a variety of biological substances, is favored by consumers at home and abroad. It is one of the rare varieties of edible fungi. Its production cycle is short and cost-effectiveness is relatively high, in recent years has expanded rapidly.
 In order to obtain high-quality, high-yield strains of Pleurotus eryngii, This study using 60Co-γ-rays to radiation on the spores, mycelia, Fruitbody of Pleurotus eryngii, observation of their mutagenic effect. Use Microscope to observation the mycelium and contrast with Control group; Observation the colony morphology of growth contrast with Control group, and do antagonistic experiment to selected mutants.
 The experimental results show that best dose of the hyphae of Pleurotus eryngii is 500 Gy; In radiation dose of 200 Gy, the spores died rate is 70%; Fruitbody semi-lethal dose is 1000 Gy. Through Antagonism Testing and Liquid Fermented Mycelia Accumulation Test , found that radiation after the strains compared with the original strain, the accumulation of mycelium difference is not obvious, but preliminary results determine the semi-lethal dose for further study on the lure Pleurotus eryngii Variable breeding provided a theoretical basis.
 
Keywords：Pleurotus eryngii; γ- Rays irradiation; Induced Mutation; mutant strain