粗毛栓菌木質素過氧化物酶的分離與純化

食品科學論文編號:SP057  論文字數:4121,頁數:07,附任務書,開題報告,中期檢查,答辯記錄

 目  錄

摘要……………………………………………………………………………………………1
關鍵詞…………………………………………………………………………………………1
Abstract………………………………………………………………………………………1
Key words…………………………………………………………………………………… 1
引言……………………………………………………………………………………………1
1材料與方法…………………………………………………………………………………1
1.1菌株………………………………………………………………………………………1
1.2主要儀器及試劑…………………………………………………………………………2
1.3培養基……………………………………………………………………………………2
1.4樣品的制備………………………………………………………………………………3
1.4.1粗酶液的制備…………………………………………………………………………3
1.4.2酶的超濾濃縮…………………………………………………………………………3
1.5硫酸銨沉淀………………………………………………………………………………3
1.6離子交換層析……………………………………………………………………………3
2結果…………………………………………………………………………………………4
2.1酶活測定…………………………………………………………………………………4
2.2洗脫曲線…………………………………………………………………………………4
3討論…………………………………………………………………………………………5
參考文獻………………………………………………………………………………………5
致謝……………………………………………………………………………………………6

粗毛栓菌木質素過氧化物酶的分離與純化
摘要：將粗毛栓菌接種在含有麥草粉的培養基中培養60d，浸提得粗酶液，經超濾濃縮、鹽析和透析，將透析液上樣于Q-Sepharose F. F.層析柱，獲得具有木質素過氧化物酶活性的組分。
關鍵詞：粗毛栓菌，木質素過氧化物酶，離子交換柱層析

Separation and Purification of Lignin Peroxidase in Trametes gallica
Abstract： Trametes gallica was cultivated in wheat straw powder for 60 days. The crude enzyme by ultrafiltrating, salting and dialysis, was applied to Q-Sepharose FF ion exchage column. The effluent with Lignin peroxidase acticity was obtained.
Key words：  Trametes gallica,  lignin peroxidase,  ion exchage chromatography