響應(yīng)面法優(yōu)化裙帶菜粗多糖提取工藝的研究

食品科學(xué)論文編號(hào):SP117  論文字?jǐn)?shù):9601,頁(yè)數(shù):23

目錄
中文摘要 I
英文摘要 II
目錄 III
1  緒論 1
1.1 裙帶菜簡(jiǎn)介 1
1.2 多糖研究的現(xiàn)狀 2
1.3 BOX-BEHNKEN試驗(yàn)設(shè)計(jì)概述 4
1.4 研究的目的和意義 4
2  實(shí)驗(yàn)部分 6
2.1 材料 6
2.1.1 原料 6
2.1.2 試劑和設(shè)備 6
2.2 實(shí)驗(yàn)方法 7
2.2.1 工藝流程 7
2.2.2 葡萄糖含量標(biāo)準(zhǔn)曲線的繪制 7
2.2.3 熱水浸提工藝條件優(yōu)化 8
3  結(jié)果與討論 10
3.1 各個(gè)乙醇濃度沉淀之間的比較 10
3.2 單因子試驗(yàn)影響研究 10
3.2.1 提取溫度對(duì)多糖的糖含量影響 10
3.2.2 提取時(shí)間對(duì)粗多糖的糖含量的影響 11
3.2.3 水料比對(duì)粗多糖的糖含量影響 11
3.2.4 pH對(duì)粗多糖的糖含量影響 12
3.3 響應(yīng)面分析方案及結(jié)果 13
4 總結(jié)與展望 17
致  謝 18
參考文獻(xiàn) 19

摘要：多糖具用抗病毒、抗凝血等多種生理活性，本文對(duì)裙帶菜中粗多糖的提取條件進(jìn)行了研究。試驗(yàn)采用甲醇對(duì)新鮮裙帶菜進(jìn)行預(yù)處理，除絕大部分的色素，用熱水浸提，采用不同濃度乙醇進(jìn)行分級(jí)沉淀,蒽酮硫酸法檢測(cè)多糖主要存在于當(dāng)乙醇濃度為60％的沉淀中。在單因子試驗(yàn)基礎(chǔ)上，選取裙帶菜粗多糖提取時(shí)間、提取溫度和料液比3個(gè)因素進(jìn)行中心組合設(shè)計(jì)，利用響應(yīng)面分析法(RSA)對(duì)粗多糖的提取工藝進(jìn)行優(yōu)化。依據(jù)回歸分析確定工藝條件的影響因子，在分析各個(gè)因素的顯著性和交互作用后，從而得出最佳水浸提工藝條件：提取溫度75.8℃、水料比6.3∶1、提取時(shí)間2.2h、提取2次，裙帶菜粗多糖的糖含量為7.73％。
關(guān)鍵詞：裙帶菜；提取；單因子試驗(yàn)；響應(yīng)面法
 
Abstract：The polysaccharide has a variety of biological activities, such as anticoagulant activities and antiviral activities. In this paper extraction conditions of Undaria pinnatifida polysaccharides were studied. The polysaccharide was extracted from fresh Undaria pinnatifida which was pretreated with methanol to get rid of most pigment by hot water, then ethanol was used to make polysaccharide precipitated. Anthrone-sulfuric acid was used to determine the content of polysaccharide and the result showed that most polysaccharide was found in the deposition when the consistence of ethanol was 60%. On the base of the single factor experiment and a three-level three-factor Box-Behnken design, the effects of different temperature, extract time and the ratio of solid to solution on the yield of the polysaccharide were investigated. The extraction technique parameters were optimized with Response Surface Methodology and the optimum conditions of extraction were as follow: the temperature 75.8℃, the extraction time 2.2h, extraction twice, ratio of water to solution 6.3∶1, In this condition, extraction rate of Undaria pinnatifida polysaccharide was 7.73%.
Keywords：Undaria pinnatifida; Extraction; Single-factor test; Response surface methodology