高脫乙酰度低分子量殼聚糖L-乳酸鹽的制備工藝研究

食品科學論文編號:SP119  論文字數:10574,頁數:26

目錄
中文摘要 ............................................................I
英文摘要 ...........................................................II
目錄 ..............................................................III
1 緒論 .......................................................................1
1.1 殼聚糖概論 .........................................................1
1.2 低聚殼聚糖的性質和應用 ......................................2
1.3 高脫乙酰度殼聚糖的制備研究進展 ..............................4
1.4 殼低聚塘制備研究進展 ........................................4
1.5脫乙酰度檢測方法的研究進展 .................................. 6
2 實驗內容 ..........................................................8
2.1實驗儀器 .....................................................8
2.2 實驗原料與試劑 ..............................................8
2.3 高脫乙酰度的殼聚糖的制備 ....................................8
2.4 殼聚糖脫乙酰度的測定方法 ....................................9
2.5 低分子量殼聚糖的制備........................................10
2.6 殼聚糖溶液分子量的測定方法 .................................11
2.7 低聚殼聚糖L-乳酸鹽的制備 ..................................11
3 結果和分析 .......................................................12
3.1 酸堿滴定法與電位滴定法的比較 ...............................12
3.2 各因素對殼聚糖脫乙酰度的影響 ...............................14
3.3 殼聚糖分子量的變化 .........................................16
總結與展望..........................................................20
致謝 ...............................................................21
參考文獻 ...........................................................22

摘要：隨著化學及其他化工業的發展及對殼聚糖研究的深入，發現殼聚糖具有許多優異的特性，越來越受到人們的重視。本課題將進行高脫乙酰度低分子量殼聚糖L-乳酸鹽的研制，目的是通過提高殼聚糖的脫乙酰度增加殼聚糖游離化銨的濃度，提高其抑菌效果；同時進行L-乳酸化，增加游離化銨的強度，增加其水溶性；而小分子量的殼聚糖也具有良好的水溶性好，容易進入細菌的細胞膜，從而提高其抑菌和殺菌的效果。本文通過使用相轉移催化劑來提高殼聚糖的脫乙酰度，使用過氧化氫降解法降低殼聚糖的分子量。高脫乙酰度低分子量殼聚糖L-乳酸鹽將具有良好的水溶性和抑菌效果。在眼科用藥等領域中具有廣闊的前景。
關鍵詞：低聚殼聚糖；高脫乙酰度；相轉移催化劑；過氧化氫降解

Abstract：With the developing of chemical industry and in-depth studying of chitosan, people found that chitosan has many excellent characteristics, which attracted more attention. In this topic prepared the chitosan L-lactic acid with high degree and low molecular of deacetylation, Aim to increase the concentration of ammonium by improving the degree of deacetylation Chitosan, and L-lactic acidifying at the same time; increasing the intensity of ammonium to further increase its water-soluble; small molecular Chitosan has good water-solubility, easy accessing to the bacterial cell membrane, thus enhancing its bacteriostatic and bactericidal effects. We used phase transfer catalyst to improve the deacetylation of Chitosan, and the hydrogen peroxide degradation to reduce the molecular of Chitosan. High degree of deacetylation and low molecular Chitosan L-lactate which has good solubility in water has good antibacterial effects. It has broad prospection in the field of ophthalmic medication.

Key  words:  low molecular weight chitosan ;  high deacetylation degree ; Phase transfer catalyst ； hydrogen peroxide depolymerization ;