液相色譜串聯質譜法測水產品中的硝基呋喃代謝物

食品科學論文編號:SP105  論文字數:6170,頁數:18

 中文摘要……………………………………………………………………………..1
英文摘要……………………………………………………………………………..1
1     緒論……………………………………………………………………………2
 1.1 前言……………………………………………………………………...2
2    試驗部分……………………………………………………………………….4
 2.1試劑和材料………………………………………….……………..… ..4
 2.2儀器和設備………………………………………………………………. 6
 2.3儀器參數與測定   ………………………………………………………  6
3    實驗方法……………………………………………………………………….7
      3.1試樣制備………………………………………………………………….7
 3.2分析步驟…………………………………………………………………7
 3.3凈化步驟 ………………………………………………………………..8
 3.4校正曲線………………………………………………………………….8
結果與分析…………………………………………………………..……….8
4.1過柱量選擇………………………………………………………………..8
4.2衍生溫度和時間的選擇……………………….………………………..10
4.3提取凈化方法的選擇…………………………….……………………..10
硝基呋喃代謝物衍生物的工作曲線及相關系數…………………………..11
注意事項……………………………………………………………………..14
回收率和精密度……………………………………………………………..14
參考文獻……………………………………………………………………..16
致謝…………………………………………………………………………..17

附錄A：標準品色譜圖

摘要：由于硝基呋喃類藥物對人類健康的潛在危害，歐盟已經禁止其在動物食品生產中使用。采用固相萃取(SPE)前處理凈化技術, 高效液相色譜-串聯質譜(LC-MS/MS)法測定水產中呋喃它酮(furaltadon)、呋喃西林(furacilin)、呋喃妥因(titrofurantoine)、呋喃唑酮(furazolidonum)代謝產物的殘留量。分析物采用多反應監測方法(MRM)，同位素內標法定量。

關鍵詞：硝基呋喃代謝物；水產品；固相萃取；高效液相色譜-串聯質譜
  
Highly effective liquid chromatography - series mass spectrography determination pesticide residues nitryl fu nan kind of antibiotic metabolite
Abstract：The use of nitrofurans in food-producing animals has been banned in EU, because they may represent a public health risk. A method has been developed to determine the pesticide residues of furaltadon, furacilin, titrofurantoine and furazolidonum in Aquatic products by LC-MS/MS coupled with the solid phase extraction(SPE).Identification was achieved by using multiple reaction monitoring(MRM). The quantification was performed with isotope as the internal standard.

Key word：nitrofuran metablolites；pesticide residues；SPE；LC-MS/MS