雙孢蘑菇褐變與多酚氧化酶活性關系的研究

生物工程論文編號:SW013  論文字數:8707,頁數:23

目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1  關于雙孢蘑菇 1
 1.2  蘑菇的營養與經濟意義 1 
 1.3  關于多酚氧化酶PPO 2
 1.4  關于蘑菇PPO的研究及其意義 3
2. 實驗部分 5
 2.1  實驗材料 5
 2.2  實驗方法 5
 2.3  實驗步驟  6
3. 結果與討論 8
 3.1   褐變度的測定結果 8
 3.2  多酚氧化酶（PPO）活性測定結果 11
 3.3  討論    14
4．總結與展望 16
致謝 17
參考文獻 18

 摘 要：本論文主要介紹了雙孢蘑菇的研究進展（包括雙孢蘑菇的營養價值與經濟意義），多酚氧化酶活性及性質，并測量了雙孢蘑菇的褐變度及其對應的多酚氧化酶（PPO）活性的變化。褐變度是指雙孢蘑菇采摘后在一定溫度下儲藏不同時間后的褐化程度，它是用測色色差計來測量的；本試驗主要以L值大小作為雙孢蘑菇褐變程度的指標,L值越大，表示顏色越白，褐變越輕。PPO催化各種酚與O2氧化為醌。本實驗是采用鄰苯二酚為底物，在磷酸鈉緩沖液PH6.5的反應體系中，PPO催化鄰苯二酚形成褐色的醌，在分光光度計410nm處使反應體系的OD值產生變化，通過OD值上升的讀數變化確定PPO的酶活大小。結果表明，雙孢蘑菇內的PPO參與了褐變過程，并與褐化程度有一定的關系。

關鍵詞：雙孢蘑菇；褐變；多酚氧化酶

Study on the relationship between agaricus bisporus browning and activity of polyphenol oxidase

Abstract: This article introduces the progress of agaricus bisporus(including the nutritional value and economic significance of agaricus bisporus), polyphenol oxidase activity and nature, and measured the agaricus bisporus browning and its corresponding level of polyphenol oxidase (PPO) activity .Browning is agaricus bisporus which after picking under a certain temperature storage at different times after the extent of browning,it is used color measurement to measure , the size of L is degreed the agaricus bisporus browning in this test, L greater value, as the color greater white and as the browning lighter. PPO catalytic various of phenol and O2 oxidation of quinine. This study was used as the substrate catechol, sodium phosphate buffer pH6.5 response system, PPO catalytic formation of catechol brown quinone, Spectrophotometer at 410nm in reaction to the OD values change, use the reading of OD value rose to determine the size of PPO activity. The results show PPO is concerned with agaricus bisporus browning process, and with a certain relationship degree of browning .

Keywords：Agaricus bisporus; Browning ; Polyphenol oxidase