烏賊內殼中β-殼聚糖的制備研究

生物工程論文編號:SW015  論文字數:10660,頁數:22

目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1  前言 1
      1.2  文獻綜述 2
2. 實驗部分 7
 2.1 實驗原理  7
 2.2 實驗原料與儀器  7
 2.3 實驗步驟 8
 2.4 實驗工藝流程 10
3. 結果與討論 11
 3.1 單因素實驗   11
 3.2 正交實驗 12
 3.3 實驗結果數據處理 13
 3.4 產物的紅外分析 14
4. 總結與展望 16
致謝 17
參考文獻 18

摘　要:選用烏賊內殼為原料，用化學方法提取出β-甲殼素，研究了將β-甲殼素進行脫乙�；�制備β-殼聚糖的最優化設計。實驗考察氫氧化鈉濃度、反應時間、反應溫度各個因素對β-殼聚糖脫乙酰度的影響，選擇了三因素三水平的正交實驗方案L9(33)。對實驗數據圖表進行分析，得出各個因素對于β-殼聚糖脫乙酰度的影響程度各不相同，其中氫氧化鈉的濃度對于實驗結果的影響最顯著，反應溫度次之，反應時間影響最小。結果表明：β-殼聚糖的脫乙酰度隨著氫氧化鈉溶液的濃度、反應溫度和反應時間的增加而增大。對正交實驗結果進行分析處理后，得出甲殼素脫乙酰的最優條件為氫氧化鈉的濃度為60%，反應溫度為110℃，反應時間為4小時。在該反應條件下得到β-殼聚糖的脫乙酰度為85.92%。
關鍵詞: 烏賊；β-甲殼素；β-殼聚糖；脫乙酰

Study on the Preparation of β-Chitosan of Sepia Shell
Abstract: Sepia shell was chosen as the raw material for the extraction of β-chitin. The optimal design of the deacetylation conditions of β-chitin which used to prepare β-chitosan was studied in this paper . The influence of the deacetylation temperature, deacetylation time, and sodium hydroxide concentration on the degree of deacetylation(DD) of deacetylated products prepared from β-chitin were discussed . A L9 (33) orthogonal table was used to complete the preparation of β-Chitosan . The results revealed that the DD values of β-Chitosan increase as the deacetylation temperature, deacetylation time, and sodium hydroxide concentration increases. Among these three factors, the sodium hydroxide concentration influenced the results significantly, and the second was the deacetylation temperature. The result showed that the most suitable and proper reaction conditions of deacetylation was that: the concentration of sodium hydroxide was 60%, the deacetylation temperature was 110℃, the deacetylation time was 4 hours, and the DD of the product was 85.92%.
Key words : Sepia; β-Chitin; β-Chitosan; Deacetylation