小兒咽扁顆粒中綠原酸的含量測(cè)定

生物工程論文編號(hào):SW021  論文字?jǐn)?shù):9104,頁(yè)數(shù):25

目  錄
中文摘要 I
英文摘要 II
目錄 III
1. 緒論 1
 1.1  小兒咽扁顆粒簡(jiǎn)介 1
 1.2  綠原酸簡(jiǎn)介 2
 1.3  高效液相色譜法測(cè)定綠原酸含量綜述 4
2. 實(shí)驗(yàn)部分 6
 2.1  實(shí)驗(yàn)原料和儀器 6
 2.2  色譜條件的選擇試驗(yàn) 6
 2.3  標(biāo)準(zhǔn)曲線的制備及線性范圍的考察試驗(yàn) 7
 2.4  精密度考察試驗(yàn) 7
 2.5  提取方法的選擇試驗(yàn) 7
 2.6  提取溶媒選擇試驗(yàn) 7
 2.7  提取時(shí)間選擇試驗(yàn) 7
 2.8  綠原酸溶液的穩(wěn)定性試驗(yàn) 7
 2.9  重復(fù)性及日間精密度試驗(yàn) 8
 2.10  回收率試驗(yàn) 8
 2.11  綠原酸含量測(cè)定方法試驗(yàn) 8
 2.12  陰性對(duì)照溶液的制備試驗(yàn) 9
 2.13  樣品的測(cè)定及含量限度的確定試驗(yàn) 9
 2.14  藥材的測(cè)定試驗(yàn) 9
 2.15  藥品標(biāo)準(zhǔn)物質(zhì)的有關(guān)資料 9
3. 結(jié)果與討論 10
 3.1  色譜條件的選擇 10
 3.2  標(biāo)準(zhǔn)曲線的制備及線性范圍的考察 11
 3.3  精密度考察 12
 3.4  提取方法的選擇 13
 3.5  提取溶媒選擇 13
 3.6  提取時(shí)間選擇 13
 3.7  綠原酸溶液穩(wěn)定性試驗(yàn) 14
 3.8  重復(fù)性及日間精密度 14
 3.9  回收率 14
 3.10  測(cè)定法 15
 3.11  樣品的測(cè)定及含量限度的確定 15
 3.12  藥材的測(cè)定 16
4. 總結(jié)與展望 17
致謝 18
參考文獻(xiàn) .19

摘 要：本論文建立小兒咽扁顆粒中綠原酸含量的測(cè)定方法。采用HPLC法測(cè)定小兒咽扁顆粒中綠原酸的含量，采用依利特Hypersil ODS柱，乙腈-0.2%磷酸溶液（10∶90）為流動(dòng)相，流速為1.0mL/min，超聲和回流提取綠原酸的效果相當(dāng)，但超聲方法簡(jiǎn)單，故選擇超聲作為提取綠原酸的方法，以50%甲醇作為提取溶媒，提取時(shí)間為20分鐘， 檢測(cè)波長(zhǎng)為327 nm。結(jié)果表明：綠原酸在1.016～101.6μg/mL之間呈良好的線性關(guān)系(γ=1)，綠原酸含量測(cè)定方法，進(jìn)樣精密度良好，綠原酸在50%甲醇中12小時(shí)之內(nèi)穩(wěn)定，綠原酸含量測(cè)定方法，重復(fù)性及日間精密度良好，平均加樣回收率為98.6％(n=6)，RSD為0.55％。本法操作簡(jiǎn)便、易行、準(zhǔn)確、重現(xiàn)性好，可用于該制劑的質(zhì)量控制。
關(guān)鍵詞：小兒咽扁顆粒；高效液相色譜；綠原酸

The Determination Of Chlorogenic Acid Content In Xiao’er Yan

Abstract:  This article is about establishing a method of quality control for assaying chlorogenic acid in Xiao’ er yan bian particle.Xiao’ er yan bian particle was analysized by HPLC.An yi li te Hypersil ODS column was used with a mobile phase consisted of mixture of acetonitrile—0.2%phosphoric acid (10：90,by volume ratio)by flowing rate of 1.0mL/min in this method.The methods of extracting chlorogenic acid by hypersound and backstreaming were equivalented.The hypersound was more convenient ,so we chose hypersound as the method to extract chlorogenic acid. Using 50% (by volume ratio) methanol as the menstruum,the time of extracting was 20 minutes.And VWD detector wavelength was 327nm.The results suggested that the linear range of chlorogenic acid was 1.016～101.6μg/mL (r= 1).The precision of sample introduction was very good by this method and chlorogenic acid was steady in 50% (by volume ratio) methanol in 12 hours . The determination reproducibility and daytime degree of precision of the method were very good.The average recovery was 98.6％ (RSD=0.55％,n=6) . In Conclusion, this was an accurate, simple, rapid qualitative method with good reproducibility to assay chlorogenic acid in Xiao’ er yan bian particle.
Keywords：Xiao’ er yan bian particle；HPLC；Chlorogenic acid