桑葚中生物類總黃酮的提取工藝及其研究

生物工程論文編號:SW032  論文字數:11089,頁數:31

目錄
中文摘要........................................................... I
英文摘要...........................................................II
目錄............................................................. III
1. 緒論.............................................................1
   1.1 前言.........................................................1
   1.2 黃酮類化合物的結構...........................................1
   1.3 背景.........................................................1
2. 實驗部分.........................................................4
   2.1 主要材料和試劑...............................................4
   2.2 實驗方法.....................................................4
3. 結果與討論.......................................................7
   3.1 單因素實驗...................................................7
   3.2 總黃酮提取正交實驗結果與分析................................11
   3.3 四種樹脂靜態吸附與解吸......................................13
   3.4 大孔吸附樹脂分離純化實驗分析................................15
   3.5 大孔樹脂的預處理與強化再生..................................22
   3.6 小結 22
4. 總結與展望 23                                             
致謝 25
參考文獻 26

摘要：本論文研究桑葚中生物類總黃酮的提取條件與大孔吸附樹脂分離與純化總黃酮的工藝條件。結合正交實驗設計方法，以桑葚中生物類總黃酮的提取率為考察指標，對影響桑葚中生物類總黃酮提取的乙醇質量分數，提取時間，提取溫度，料液比進行研究；以桑葚中總黃酮吸附量、解吸量為指標，選擇合適的大孔吸附樹脂，并研究吸附與解吸優化條件，對影響分離純化的pH值，無機鹽濃度，溫度，乙醇濃度等進行了分析。桑葚中生物類總黃酮提取的最優條件，即:以75%乙醇為提取劑，提取溫度75℃，料液比為1:15,提取時間為2 h，再結合分離純化的較優條件，條件為:待分離樣品溶液pH值調至3.0-4.0左右，無機鹽濃度調至3%-6%，吸附的溫度為50℃，洗脫最佳溶劑為50％的乙醇溶液，洗脫速率為0.5-1.0ml/min,洗脫用量為3倍的柱體積。桑葚中生物類總黃酮的提取率可以達到 2600mg/kg（以蘆丁計）,桑葚提取物總黃酮的含量接近50%。此正交實驗設計可行，選取D-101型樹脂為合適大孔吸附樹脂，選取藥桑葚或黑桑葚為佳,D-101型大孔吸附樹脂適用于桑葚中總黃酮的分離與純化。

關鍵詞：桑葚，總黃酮，正交設計，大孔吸附樹脂，分離純化
 Extraction Condition and Research of Total
 Flavonoids in Mulberry

Abstract: To study technique condition for separating total flavonoids from mulberry. With orthogonal experiment based on single factor, The total extraction rate of flavonoids for purpose, the effects of ethanol concentration, ratio of material to liquid, extraction temperature, extraction temperature, extraction time and times on extraction of total flavonoids from mulberry were study to obtain the optimal extraction conditions; adsorbance of mulberry total flavonoids for indicators, Choose a suitable macroporous resin, Study Adsorption and Desorption optimum conditions, analysis pH, salt concentration, temperature, ethanol concentration. Mulberry of total flavonoids extracted from optimal conditions, 75% of ethanol, water bath temperature of 75 ℃, extracting liquid ratio of 1:15, extraction time is 2h, pH is 4.0 , salt oconcentration is 3% , temperature is 50℃, ethanol concentration is 50%, elution rate of ethanol is 0.5-1.0ml/min,3 column volume. Mulberry of total flavonoids in the extraction rate can be achieved 2600mg/kg. Under the experimental conditions, total flavonoids extracted from mulberry by D-101 macroporous resin nearly 50%. Choose the D-101macroporous resin, choose the medicine mulberry or black mulberry, and D-101 macroporous resin applied to mulberry of total flavonoids in the separation and purification. 

Keywords: Mulberry, total flavonoids, orthogonal design, macroporous resin, separation and purification.