高產(chǎn)SOD菌株HB25的發(fā)酵條件優(yōu)化研究

生物工程論文編號(hào):SW048  論文字?jǐn)?shù):11143,頁(yè)數(shù):22

目  錄
中文摘要 I
英文摘要. II
目  錄 Ⅲ
1．緒論 1
1.1 SOD的種類及分布 1
1.2 SOD的催化機(jī)理及活性中心 2
1.3 SOD的主要應(yīng)用 3
1.4 國(guó)內(nèi)外研究概況 5
1.5 本項(xiàng)目的研究目的及意義 6
2．實(shí)驗(yàn)部分 7
2.1 材料與方法 7
2.2 試驗(yàn)設(shè)計(jì) 8
3．結(jié)果與討論 10
3.1 發(fā)酵工藝條件實(shí)驗(yàn)結(jié)果 10
3.2 培養(yǎng)基組成單因子條件試驗(yàn)結(jié)果 11
3.3 優(yōu)化培養(yǎng)條件的結(jié)果 13
3.4 小結(jié) 13
4．結(jié)論與展望 14
致謝 15
參考文獻(xiàn) 16

摘  要：采用正交試驗(yàn)及單因子條件實(shí)驗(yàn)對(duì)菌株HB25進(jìn)行發(fā)酵產(chǎn)酶的影響因素及發(fā)酵工藝條件進(jìn)行了研究。結(jié)果表明：其最佳培養(yǎng)基組成為葡萄糖2%，蛋白胨2%，酵母膏1%，7Be麥芽汁9%（V/V），CuSO475mmol/ml，ZnSO425mmol/ml ；最佳培養(yǎng)條件為：初始pH5.5,搖瓶裝量為75 ml/500 ml三角瓶，接種量為15%，28C培養(yǎng)20小時(shí)，加H2O2使終濃度為1%，繼續(xù)培養(yǎng)至24小時(shí)。在此條件下，該菌細(xì)胞生物量為5.26g/100ml，SOD含量為2662u/g濕菌體，SOD產(chǎn)量為 1.40104u/100ml，SOD產(chǎn)量較出發(fā)菌株提高99%。
關(guān)鍵詞：菌株HB25；發(fā)酵條件；優(yōu)化

Studies on Optimizing fermentation condition of strain HB25

Abstract: Based on orthogonal and monofactorial test, the medium and fermentation condition was studied. It showed the optimal medium consisted of 2% glucose, 9%(V/V) malt wort, 2%peptone, 1%yeast extract, CuSO475mmol/ml, ZnSO4 25mmol/ml; the optimal culture condition were as following: Initial pH 5.5, medium cubage was 75ml/500ml, 15% concentration was inoculated; After 20 hours, H2O2  was added to the 1% final concentration, then cultured to 24 hours. Under this condition, the biomass was 5.26g/100ml, SOD activity was 2662u/g fresh cell, SOD production was 1.40104 u/100ml, which was 99% higher than original strain.

Key words: Strain HB25; Fermention condition; Optimization